CORE--MACROMOLECULAR LABORATORY RESOURCE

核心--高分子实验室资源

• 批准号: 6573848
• 负责人: THOMAS L CIARDELLI
• 金额: $ 24.84万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6573848
• 关键词: biomedical facility; computer assisted sequence analysis; nucleic acid chemical synthesis; nucleic acid sequence; oligonucleotides; protein sequence; synthetic nucleic acid

Description: (Applicant?s Description) The Molecular Biology Laboratory (MB Lab) is a Shared Resource for macromolecular (DNA, RNA, protein) synthesis and sequencing which is run as a Shared Resource by the Cancer Center. The MB Lab is vital to the research mission of the Cancer Center, providing services in a highly cost-effective way for its members and enabling them to perform experiments that might not otherwise be possible or practical. The current MB Lab occupies a total of approximately 600 net sq. ft. in the Vail Building of Dartmouth Medical School. This facility currently has the following key equipment: two DNA synthesizers (a BioSearch Cyclone model 8400 dual column and an Applied Biosystems Inc. (ABI model 392 four column); two DNA sequencers (ABI model 373A stretch); and a protein sequencing system (ABI model 476A). A peptide synthesizer (ABI model 431A) is also available for the Resource?s?s use at the nearby VA Medical Center in White River Junction VT. The Resource also manages two phosphorimaging and densitometry systems (Molecular Dynamics, one on each campus), and a BIAcore biosenser apparatus (Pharmacia), and is responsible for the x-ray film processor equipment (three instruments on two campuses). Support equipment includes two HPLC systems (Hewlett Packard 1050 and 1090L), two speed-vac systems (Savant), and miscellaneous necessary small equipment. The Resource currently has three technical personnel i.e., a manager, a technical specialist, and a technical assistant. The MB Lab is run as a cost center, and services are provided to the greater Dartmouth research community using a fee-for-services charge-back system. Prices are competitive with outside vendors where applicable, and several services are unavailable from outside contractors. This program is managed by a faculty director, Dr. Joshua Hamilton, and is overseen by a MB Lab Advisory Committee. The financial and other administrative responsibilities of the MB Lab Shared Resource are managed by the Cancer Center. Funding for the current MB Lab budget is provided by the Cancer Center, the Dartmouth College Superfund Basic Research Program Project, the Resource charge-back system, and institutional funds from Dartmouth College and Dartmouth Medical School. Currently 51 (78 percent) of the 65 laboratories within the greater Dartmouth-Hitchcock research community that use the MB Lab are those of Cancer Center peer-reviewed funded members.

描述：(申请人？S描述)分子生物学实验室(MB Lab)是合成大分子(DNA、RNA、蛋白质)的共享资源 由癌症中心作为共享资源运行的测序。MB实验室 对癌症中心的研究任务至关重要，在 为其成员提供极具成本效益的方式，使他们能够 否则可能是不可能或不可行的实验。当前MB 实验室总占地面积约600平方米。英国《金融时报》在Vail大楼里 达特茅斯医学院。此设施当前具有以下密钥 设备：两台DNA合成器(BIOSARCH旋风8400型双柱 和一台应用生物系统公司(ABI型号392四柱)；两台DNA测序仪 (ABI型号373A拉伸)和蛋白质测序系统(ABI型号476A)。一个 多肽合成仪(ABI型431A型)也可用于资源？S？S 在附近的弗吉尼亚州怀特河交界处的退伍军人医疗中心使用。《资源》 还管理两个磷成像和密度测量系统(分子动力学、 每个校区一个)和一个Biacore生物传感器设备(Pharmacia)，并且是 负责X光胶片处理机设备(三台仪器上两台 校园)。支持设备包括两个高效液相系统(惠普1050 和1090L)，两个变速真空系统(Savant)，以及其他必要的小 设备。该资源目前有三名技术人员，即一名 经理、技术专家和技术助理。正在运行MB实验室 作为成本中心，并为大达特茅斯研究中心提供服务 社区使用按服务收费的收费系统。价格极具竞争力 在适用的情况下与外部供应商合作，但有几项服务不可用 从外部承包商那里。该项目由一位教员主任Dr。 约书亚·汉密尔顿，并由MB实验室咨询委员会监督。这个 分担甲基溴实验室的财务和其他管理责任 资源由癌症中心管理。为目前的甲基溴实验室提供资金 预算由达特茅斯学院超级基金癌症中心提供 研究计划项目、资源收费制度和制度 资金来自达特茅斯学院和达特茅斯医学院。目前51(78 在大达特茅斯-希区柯克地区内的65个实验室中 使用MB实验室的研究社区是癌症中心的社区 同行评议的资助成员。