CORE--MACROMOLECULAR LABORATORY RESOURCE

描述：（申请人？分子生物学实验室（MB） 实验室）是大分子（DNA，RNA，蛋白质）合成的共享资源， 这是由癌症中心运行的共享资源。MB实验室 对癌症中心的研究使命至关重要， 以成本效益高的方式，使其成员能够履行 这些实验本来是不可能或不切实际的。当前MB 实验室占地约600平方米。英尺在维尔大厦， 达特茅斯医学院。此设施当前具有以下密钥 设备：两台DNA合成仪（BioSearch Cyclone 8400型双柱 和应用生物系统公司(ABI 392型四柱）;两台DNA测序仪 (ABI 373 A型延伸）;和蛋白质测序系统（ABI 476 A型）。一 肽合成仪（ABI型号431 A）也可用于资源？是什么？S 在附近的白河交汇处VT的VA医疗中心使用。资源 还管理两个磷光成像和密度测定系统（分子动力学， 每个校区一个）和BIAcore生物传感器装置（Pharmacia）， 负责X光胶片冲洗设备（三台仪器上两台 校园）。支持设备包括两个HPLC系统（Hewlett Packard 1050 和1090 L），两个速度真空系统（Savant），以及各种必要的小型 设备.该资源目前有三名技术人员，即：一 经理、技术专家和技术助理。MB实验室运行 作为成本中心，并为更大的达特茅斯研究提供服务 社区使用按服务收费的收费系统。价格有竞争力 在适用的情况下与外部供应商合作，并且无法获得几项服务 从外部承包商。该项目由学院院长Dr. 约书亚汉密尔顿，并由MB实验室咨询委员会监督。的 MB实验室的财务和其他行政责任 资源由癌症中心管理。当前MB实验室的资金 预算是由癌症中心，达特茅斯学院超级基金基本 研究计划项目、资源收费系统和机构 来自达特茅斯学院和达特茅斯医学院的资金。51（78） 在达特茅斯-希区柯克地区的65个实验室中， 使用MB实验室的研究社区是癌症中心的研究社区 同行评审的资助成员。

Description: (Applicant?s Description) The Molecular Biology Laboratory (MB Lab) is a Shared Resource for macromolecular (DNA, RNA, protein) synthesis and sequencing which is run as a Shared Resource by the Cancer Center. The MB Lab is vital to the research mission of the Cancer Center, providing services in a highly cost-effective way for its members and enabling them to perform experiments that might not otherwise be possible or practical. The current MB Lab occupies a total of approximately 600 net sq. ft. in the Vail Building of Dartmouth Medical School. This facility currently has the following key equipment: two DNA synthesizers (a BioSearch Cyclone model 8400 dual column and an Applied Biosystems Inc. (ABI model 392 four column); two DNA sequencers (ABI model 373A stretch); and a protein sequencing system (ABI model 476A). A peptide synthesizer (ABI model 431A) is also available for the Resource?s?s use at the nearby VA Medical Center in White River Junction VT. The Resource also manages two phosphorimaging and densitometry systems (Molecular Dynamics, one on each campus), and a BIAcore biosenser apparatus (Pharmacia), and is responsible for the x-ray film processor equipment (three instruments on two campuses). Support equipment includes two HPLC systems (Hewlett Packard 1050 and 1090L), two speed-vac systems (Savant), and miscellaneous necessary small equipment. The Resource currently has three technical personnel i.e., a manager, a technical specialist, and a technical assistant. The MB Lab is run as a cost center, and services are provided to the greater Dartmouth research community using a fee-for-services charge-back system. Prices are competitive with outside vendors where applicable, and several services are unavailable from outside contractors. This program is managed by a faculty director, Dr. Joshua Hamilton, and is overseen by a MB Lab Advisory Committee. The financial and other administrative responsibilities of the MB Lab Shared Resource are managed by the Cancer Center. Funding for the current MB Lab budget is provided by the Cancer Center, the Dartmouth College Superfund Basic Research Program Project, the Resource charge-back system, and institutional funds from Dartmouth College and Dartmouth Medical School. Currently 51 (78 percent) of the 65 laboratories within the greater Dartmouth-Hitchcock research community that use the MB Lab are those of Cancer Center peer-reviewed funded members.