CORE--FLOW CYTOMETRY AND FLUORESCENCE IMAGING FACILITY

描述：（申请人描述）流式细胞术/荧光 成像共享资源为癌症中心研究人员提供先进的 研究细胞的仪器。为此，它旨在提供 最先进的流式细胞仪和图像分析系统， 数据;为细胞分析提供计算机硬件和软件 从这些仪器获得的数据;培训科学家使用 仪器和软件;开发和实施新技术， 分析;并鼓励科学家之间就共同的研究方法进行交流。 兴趣 对于流式细胞术，共享资源有两台Becton Dickinson仪器： FACStar Plus维斯/UV七参数流式细胞仪（配备TurboSort升级版）， 分选和先进的应用程序和FACScan五参数细胞仪， 常规分析资源实验室还有三个图像分析 系统：Bio-Rad MRC-1024氪/氩激光共聚焦系统; Meridian Ultima UV/维斯氩激光共聚焦系统;和Zeiss Axiophot 带有冷却CCD探测器的显微摄影机。其中，在共享 资源，一台Silicon Graphics工作站，三台联网的Macintosh 工作站和一台Codonics热升华打印机， 彩色印刷 在1996 - 1997财政年度，流式细胞术/荧光成像共享资源 由来自34个实验室的大约100个不同的人使用 investigators.在此期间，在总共2 858个计费小时中， 癌症中心成员使用（占总计费使用量的95.9%）。在 目前，癌症中心的研究人员使用共享资源， 每年直接费用共计10，399，757美元的积极赠款。其中，97 %来自同行评审赠款（84.2%来自NIH）。

Description: (Applicant's Description) The Flow Cytometry/Fluorescence Imaging Shared Resource provides Cancer Center investigators with advanced instrumentation for the study of cells. To this end, it aims to provide state-of-the-art flow cytometers and image analysis systems for acquisition of data; to provide computer hardware and software for the analysis of cellular data derived from these instruments; to train scientists in use of the instruments and software; to develop and implement new techniques for cell analysis; and to encourage communication among scientists on methods of common interest. For flow cytometry, the Shared Resource has two Becton Dickinson instruments: a FACStar Plus VIS/UV seven-parameter cytometer with TurboSort upgrade for sorting and advanced applications and a FACScan five-parameter cytometer for routine analysis. The Resource laboratory also has three image analysis systems: a Bio-Rad MRC-1024 krypton/argon laser confocal system; a Meridian Ultima UV/VIS argon laser confocal system; and a Zeiss Axiophot photomicroscope with a cooled CCD detector. There are, within the Shared Resource, a Silicon Graphics workstation, three networked Macintosh workstations, and a Codonics dye-sublimation printer for publication-quality color printing. During FY 96-97, the Flow Cytometry/Fluorescence Imaging Shared Resource was used by approximately 100 different individuals from the labs of 34 investigators. For that period, 2,741 out of a total of 2,858 billed hours of use were by Cancer Center members (95.9 percent of total billed use). At the present time, Cancer Center investigators using the Shared Resource hold active grants totaling $10,399,757 in annual direct costs. Of this sum, 97 percent is from peer-reviewed grants (84.2 percent from NIH).