QUANTITATIVE IN SITU HYBRIDIZATION--HIV1 RNA CLEARANCE FROM LYMPHOID TISSUE

定量原位杂交--从淋巴组织中清除 HIV1 RNA

• 批准号: 6566710
• 负责人: DAVID M ASMUTH
• 金额: $ 27.93万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6566710
• 关键词: AIDS therapy; antiAIDS agent; biopsy; blood chemistry; clearance rate; clinical research; cytokine; human immunodeficiency virus 1; human subject; human therapy evaluation; immunopathology chemotherapy; in situ hybridization; lymph nodes; lymphocyte; neutrophil; serum; tissue /organ preservation; tumor necrosis factor alpha; virus RNA; virus infection mechanism

These experiments have sought to better understand the pathogenesis of HIV infection by examining multiple characteristics of infected cells in both the peripheral blood and lump node tissue. The lymphoid organs serve as a protected reservior of HIV infection where virus resides even when no virus can be found in the blood of patients on successful antiretroviral therapy. These studies have compared the two compartments for the amount of virus that is present, the relationships of populations of cells, and other features of the cells such as their level of immune stimulation and the number of receptors on their surface which permits HIV entry into the cell. Subjects undergo excisional lymph node biopsy and have samples of peripheral blood taken at least two time points; one prior to initiating a new antiretroviral therapy and at a later date after 6 to 18 months of therapy. It is anticipated that new insights into how antiretroviral therapy controls HIV infection will be gained and that this knowledge can be applied to novel approaches to treating AIDS.

这些实验试图通过检查外周血和肿块结节组织中受感染细胞的多种特征来更好地理解HIV感染的发病机制。淋巴器官是HIV感染的受保护的储存库，即使在成功接受抗逆转录病毒治疗的患者血液中没有发现病毒时，病毒也存在于其中。 这些研究比较了两个隔室中存在的病毒量，细胞群体的关系，以及细胞的其他特征，如免疫刺激水平和表面上允许HIV进入细胞的受体数量。 受试者接受切除淋巴结活检，并在至少两个时间点采集外周血样本;一个时间点在开始新的抗逆转录病毒治疗之前，另一个时间点在治疗6至18个月后。 预计将获得关于抗逆转录病毒疗法如何控制艾滋病毒感染的新见解，这些知识可应用于治疗艾滋病的新方法。