PRODUCTION SEQUENCING OF DROSOPHILA GENOME

该提案要求提供资金，以扩大专门用于测序的工作 果蝇基因组 雄心勃勃的扩大规模是我们计划的一部分， 完成了120兆碱基的常染色体基因组序列， 这个中心模式有机体在世纪之交后不久。 是 预计测序产量的增加将来自于 更多的人在项目上工作，并从一个稳定的 提高测序效率。 提高的效率将是 主要是通过生物学的跨学科相互作用， LBL的自动化和计算组。 本节建议 描述了正在使用的定向策略， 自动化和计算的努力，劳动力 是有组织的，以及与生产工作相关的成本。

This proposal requests funding to expand the effort dedicated to sequencing the Drosophila genome. The ambitious scale-up is part of our plan to complete the sequence of the 120 megabases of the euchromatic genome of this central model organism shortly after the turn of the century. It is expected that the increase in sequencing production will come from both the additional number of people working on the project and from a steadily increasing sequencing efficiency. The increased efficiency will be achieved largely through the interdisciplinary interaction of the biology, automation, and computation groups at LBL. This section of the proposal describes the directed strategy that is being used, the current status of the automation and computation efforts, the manner in which the workforce is organized, and the costs associated with the production effort.