PRODUCTION SEQUENCING OF DROSOPHILA GENOME

该提案要求提供资金，以扩大致力于测序的工作 果蝇的基因组。雄心勃勃的扩大规模是我们计划的一部分 全长120兆碱基的常染色质基因组序列 这个中心模式生物在世纪之交后不久。它是 预计测序产量的增加将来自 更多的人在项目上工作，从一个稳定的 提高测序效率。提高的效率将是 这在很大程度上是通过生物学的跨学科互动实现的， 自动化，以及LBL的计算组。建议书的这一部分 描述正在使用的定向策略、 自动化和计算的努力，劳动力的方式 是有组织的，以及与生产工作相关的成本。

This proposal requests funding to expand the effort dedicated to sequencing the Drosophila genome. The ambitious scale-up is part of our plan to complete the sequence of the 120 megabases of the euchromatic genome of this central model organism shortly after the turn of the century. It is expected that the increase in sequencing production will come from both the additional number of people working on the project and from a steadily increasing sequencing efficiency. The increased efficiency will be achieved largely through the interdisciplinary interaction of the biology, automation, and computation groups at LBL. This section of the proposal describes the directed strategy that is being used, the current status of the automation and computation efforts, the manner in which the workforce is organized, and the costs associated with the production effort.