Core--RNA expression profiling

RNA表达谱核心。通过使用DNA微阵列分析，对不同细胞类型中RNA表达的全面分析已经发生了革命性的变化。这种方法可以用少量细胞或组织定量评估数千个基因的表达模式。该分析试图在体外和体内分析中为每个目标中研究的模型开发遗传标记，这些分析构成了该建议的基础。然后可以使用这些标记的鉴定来促进这些模型的假设的验证。RNA标记的使用应该是确定细胞表达谱的可靠方法。一旦鉴定了一组标志物，这些序列就可以通过另外的方法（例如，原位杂交）以进一步检验假设。核心E将与项目1和2的研究人员合作，从培养的细胞和实验操作的血管组织中鉴定标记物，这些标记物可以与核心D中从人类GA和对照动脉中制备的RNA进行比较。

RNA Expression Profiling Core. A comprehensive analysis of RNA expression in distinct cell types has been revolutionized by the use of DNA microarray analysis. This methodology can assess the expression patterns of thousands of genes in quantitative terms with small amounts of cells or tissue. This analysis seeks to develop genetic markers for the models under study in each aim in the in vitro and in vivo analyses that form the basis of this proposal. Identification of such markers can then be used to facilitate the validation of the hypotheses of these models. The use of RNA markers should be a reliable method for defining cellular expression profiles. Once a set of markers has been identified these sequences can then be used by additional methods (e.g., in situ hybridization) to further test the hypotheses. Core E will work with investigators in Projects 1 and 2 to identify markers from cultured cells and from experimentally manipulated vascular tissues that can be compared with RNA prepared from human GA and control arteries in Core D.