Core--RNA expression profiling

核心--RNA表达谱

• 批准号: 6659336
• 负责人: ALFRED LM BOTHWELL
• 金额: $ 17.75万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6659336
• 关键词: RNA; biomedical facility; blood vessels; gene expression; genetic markers; microarray technology

RNA Expression Profiling Core. A comprehensive analysis of RNA expression in distinct cell types has been revolutionized by the use of DNA microarray analysis. This methodology can assess the expression patterns of thousands of genes in quantitative terms with small amounts of cells or tissue. This analysis seeks to develop genetic markers for the models under study in each aim in the in vitro and in vivo analyses that form the basis of this proposal. Identification of such markers can then be used to facilitate the validation of the hypotheses of these models. The use of RNA markers should be a reliable method for defining cellular expression profiles. Once a set of markers has been identified these sequences can then be used by additional methods (e.g., in situ hybridization) to further test the hypotheses. Core E will work with investigators in Projects 1 and 2 to identify markers from cultured cells and from experimentally manipulated vascular tissues that can be compared with RNA prepared from human GA and control arteries in Core D.

核糖核酸表达分析核心。通过使用DNA微阵列分析，对不同类型细胞中RNA表达的全面分析已经发生了革命性的变化。这种方法可以用少量的细胞或组织来定量地评估数千个基因的表达模式。这一分析试图在体外和体内分析中为在每个目标下研究的模型开发遗传标记，形成这一提议的基础。然后，可以使用这些标记的识别来促进对这些模型的假设的验证。RNA标记的使用应该是确定细胞表达谱的可靠方法。一旦确定了一组标记，这些序列就可以通过其他方法(例如，原位杂交)来进一步检验假设。核心E将与项目1和2中的研究人员合作，从培养细胞和实验操作的血管组织中识别标记，这些标记可以与从核心D中的人GA和对照动脉制备的RNA进行比较。