Core--Histotechnology

组织技术核心将为四个项目中的每一个项目提供广泛的服务。这些服务可以分为三个特定领域，用于分析体内血管生成模型。首先，在组织采购方面提供服务，包括血管形态分析所需的专门固定和灌注固定。在第二个方面，处理收获的组织用于常规组织学和广泛的免疫组织化学技术。最后，通过使用电子显微镜和共聚焦成像，提供此类组织学和免疫组织化学成像服务。这些成像技术还包括统计形态分析和数字图像分析。因此，核心设施将为利用体内模型的项目提供全方位的服务，由一名PI和技术支持人员进行监督，以标准化程序并保持质量控制，并促进与威尔医学院广泛的成像设施的互动。此外，这将大大节省免疫组织化学用品、组织制备和切片，并降低成像设施的费用。

The histotechnology core will function to provide extensive services to each of the four projects. The services can be grouped in three specific areas, which are utilized in the analysis of in vivo models of angiogenesis. First, services are provided in the procurement of tissue, including specialized fixation and perfusion fixation needed for vessel morphometric analysis. In the second aspect, harvested tissues are processed for routine histology, and a wide range of immunohistochemical techniques. Lastly, service is provide in the imaging of such histology and immunohistochemistry, by the use of electron microscopy and confocal imaging. These imaging techniques also include statistical morphometric analysis and digital image analysis. Thus, the core facility will provide a complete range of services for projects utilizing in vivo models, with oversight by a single PI and technical support staff to standardize procedures and maintain quality control, and to facilitate interactions with the extensive imaging facilities at Weill Medical College. Additionally, this will result in significant savings in terms of immunohistochemical supplies, and in tissue preparation and sectioning, as well as reduced rates for the imaging facility.