Mechanisms and Therapy of Ischemic Sudden Cardiac Arrest

要提高由缺血和梗塞引起的心脏骤停的存活率，这是一个极其健康的问题，需要更好地了解引起心脏骤停的心律失常的机制以及在复苏尝试中遇到的问题。本计划项目申请提出在动物体内使用光电和光学测绘技术来获取这些信息。项目1(对猝死的缺血模型的慢性监测)将从长期测量的动物身上进行遥测，并使用电子标测来了解在缺血和梗塞时猝死是如何发生的，何时发生以及为什么发生。项目2(缺血和梗塞中室颤维持的机制)将使用光学和电学标测来量化维持室颤的机制。项目3(浦肯野--缺血和梗塞中的心肌折返)将使用电标测技术来研究浦肯野纤维在引发心律失常中的作用。项目4(缺血性心律失常期间的除颤机制)将使用电标测来确定复苏期间缺血时除颤升高和复颤发生率高的机制。项目5(无脉搏电活动的电疗法)将使用电和最佳标测来研究新的电疗法如何在复苏期间消除无脉搏电活动。核心A(行政)将为该项目管理和提供生物统计和手稿准备支助。核心B(仪器和软件)将提供和软件支持。核心C(动物模型和病理学)将确保最佳的动物护理和病理检查。

Increasing survival from sudden cardiac arrest caused by ischemia and infarction, an extremely health problem, requires better understanding of the mechanisms both of the arrhythmias causing it and of the problems encountered during resuscitation attempts. This program project application proposes to use electrical and optical and optical mapping techniques in animals to obtain this information. Project 1 (Chronic monitoring of ischemic models of sudden death) will telemeter from chronically instrumented animals and use of electrical mapping to understand how, when, and why sudden death occurs with ischemia and infarction. Project 2 (Mechanism of VF maintenance in ischemia and infarction) will use optical and electrical mapping to quantify the mechanisms maintaining VF. Project 3 (Purkinje-myocardial reentry in ischemia and infraction) will use electrical mapping to investigate the role of Purkinje fibers in initiating arrhythmias. Project 4 (Defibrillation mechanisms during ischemic arrhythmias) will use electrical mapping to determine the mechanisms for the elevated defibrillation and high incidence of refibrillation in ischemia during resuscitation. Project 5 (Electrical therapy for pulseless electrical activity) will use electrical and optimal mapping to study how new electrical therapies eradicate pulseless electrical activity during resuscitation. Core A (Administration) will administrate and provide biostatistical and manuscript preparation support for the project. Core B (Instrumentation and Software) will provide and software support. Core C (Animal models and pathology) will insure optimal animal care and pathological examination.