HIGH EFFICIENCY MAPPING OF ALCOHOL SENSITIVITY GENES

我们建议从长睡眠（ILS）和短睡眠（ISS）自交系中构建大量重组自交系（RIs），并绘制与酒精作用有关的8个性状的遗传图谱。这个新生成的RI系列将在几十种不同的行为分析中有用，而只需要进行一组基因型评估。从单一的基因型评估将流定位结果适用于无数不同的表型，已经评估了亲本品系（ILS和ISS）或LS和SS选择系。主要的缺点。通过使用如此大的菌株集，克服了与RI菌株相关的问题（在QTL鉴定中缺乏能力和在制图中缺乏分辨率）。由科罗拉多大学和行为遗传学研究所的内部资源资助，RI的构建已经开始，我们建议将这些RI携带到F22，这将允许98%以上的固定。在这个项目完成后，我们将创建一个电子数据库，让所有酒精研究人员都能方便地访问我们的所有结果。我们还将开始安排将这些老鼠分发给希望使用它们的搜索者。

We propose to complete the construction of a large number of recombinant inbred strains (RIs) from the inbred Long Sleep (ILS) and the inbred Short Sleep (ISS) strains and to map genetically eight traits involved in alcohol action. This newly generate RI series will be useful in dozens of different behavioral analyses, while only one set of genotypic assessment swill need to be performed. From the single assessment of genotype will flow mapping results applicable to a myriad of various phenotypes that have been assessed in the parental strains (ILS and ISS) or in the LS and SS selected lines. The major disadvantages. associated with RI strains (lack of power in QTL identification and lack of resolution in mapping) are overcome through the use of such a large set of strains. The RI construction has already begun, funded by internal resources of the University of Colorado and the Institute for Behavioral Genetics and we propose to carry these RIs to the F22, which will allow greater than 98% fixation. At the completion of this project we will create an electronic data base allowing all alcohol researchers easy access to all of our results. We will also begin making arrangements to distribute these mice to searchers who wish to use them.