Molecular Biophysics of Myosin and Actin in Aged Muscle

衰老肌肉中肌球蛋白和肌动蛋白的分子生物物理学

• 批准号: 6649701
• 负责人: DAWN A LOWE
• 金额: $ 9.92万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6649701
• 关键词: actins; age difference; aging; biophysics; electron spin resonance spectroscopy; laboratory rat; molecular dynamics; muscle; muscle function; myosins; nanotechnology; protein protein interaction; protein structure; protein structure function

DESCRIPTION (provided by applicant): Applicant: The applicant, Dawn A. Lowe, is a physiologist whose research is focused on molecular mechanisms underlying age-induced deterioration of muscle. Although trained in muscle physiology, Dr. Lowe has acquired experience in muscle biochemistry and molecular biology, and in the current application proposes to learn and utilize molecular biophysical techniques to round out a multidisciplinary approach to be used in studying aging muscle. Her career goal is to develop a) an independent line of research in an academic setting, b) an extramurally-funded laboratory and c) mentoring skills needed to guide graduate students and post-doctoral fellows in research and academia. The Mentored Research Scientist Development Award will provide the necessary support and training for the applicant to reach these career goals. Career Development/Training Plan: The primary element of the plan will be the acquisition of advanced molecular biophysical techniques from mentored training. The majority of the training will come from the sponsor but key mentoring will also be obtained from an advisory committee composed of established investigators in the fields of biophysics and aging. Structured activities for further career and research development will include attendance at a summer research workshop on aging, formal course work, attendance and participation at national scientific meetings, regular involvement in journal clubs and seminars, and continued affiliation with The Center on Aging and The Center for Muscle and Muscle Disorders at the University of Minnesota. Research Plan: The overall aim of this proposal is for the Dr. Lowe to learn and apply molecular biophysical techniques to explore age-related alterations in muscle contractility. A decline in muscle function is a common yet undesired consequence of aging and in order to establish methods to reverse or prevent the decline, the underlying molecular mechanisms must first be elucidated. Dr. Lowe will use electron paramagnetic resonance spectroscopy (Aim 1) and single molecule biophysical (Aim 2) techniques to study the effects of age on the structure, function, and interaction of two fundamental contractile proteins in muscle, myosin and actin. Environment: The environment for Dr. Lowe's training plan is outstanding. The sponsor, Dr. David Thomas, is an internationally known scientist in the field of muscle biophysics, is on the Board of Directors for both of the Centers mentioned above, and is the director of two NIH-funded training programs, one in muscle and the other in biophysics.

申请人Dawn a . Lowe是一名生理学家，主要研究年龄引起的肌肉退化的分子机制。虽然在肌肉生理学方面受过训练，Lowe博士获得了肌肉生物化学和分子生物学方面的经验，并在目前的应用中提出学习和利用分子生物物理技术来完善用于研究衰老肌肉的多学科方法。她的职业目标是发展a)在学术环境下的独立研究路线，b)外部资助的实验室，c)指导研究生和博士后研究和学术界所需的指导技能。指导研究科学家发展奖将为申请人提供必要的支持和培训，以实现这些职业目标。