ANTIOXIDANT PROTECTION IN AGE-ASSOCIATED ATHEROSCLEROSIS

年龄相关动脉粥样硬化的抗氧化保护

• 批准号: 6629727
• 负责人: SARA G CARLSON
• 金额: $ 8.19万
• 依托单位: CLEVELAND CLINIC FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至 2004-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6629727
• 关键词: BCL2 gene /protein; aging; antioxidants; apoptosis; atherosclerosis; atherosclerotic plaque; dietary supplements; free radical oxygen; gene expression; glutathione; immunocytochemistry; laboratory mouse; lipid peroxides; nutrition of aging; nutrition related tag; oxidation reduction reaction; oxidative stress; pathologic process; postdoctoral investigator; selenium; terminal nick end labeling; tissue /cell culture; tocopherols; vascular endothelium

DESCRIPTION (from application): The Mentored Research Scientist Development Award would allow Dr. Sara G. Carlson, PhD, to make a successful transition into conducting independent research in the field of cardiovascular aging. Since Dr. Carlson has a solid foundation in scientific training, it is reasonable to project that toward the end of the mentored research term, she will have developed a significant body of age-related data that will form a basis for independent research status and funding. The objectives of this application are to enable Dr. Carlson to steer her current efforts toward aging research: the research career development plan consists of Dr. Carlson assuming the primary role in conducting this research project, taking course work in scientific integrity, participating in seminars, journal clubs, workshops, and scientific meetings; reading and discussing original research articles in oxidative damage and atherosclerosis; and meeting with mentors and consultants trained in aging, cardiovascular disease, and apoptosis research. The aims of the research are: 1) to identify specific steps in oxysterol- induced apoptotic pathways that are modulated by altered ROS generation and antioxidants in cultured vascular cells, and 2) to characterize age-associated atherosclerotic lesion development in the senescence-accelerated mouse (SAM) model, and explore the extent to which antioxidant supplementation reduces apoptosis during age-associated atherosclerotic lesion formation. These studies will allow the candidate to test the overall hypothesis that antioxidants exert protective effects in the atherosclerotic processes accompanying aging through inhibition of ROS-mediated apoptosis.

描述（来自应用程序）：指导研究科学家 发展奖将允许博士萨拉G。Carlson博士，为了成功地 过渡到在心血管领域进行独立研究 衰老 由于卡尔森博士在科学训练方面有着坚实的基础， 合理的项目，对指导研究学期结束时，她 将开发出大量与年龄相关的数据， 独立研究状况和资金的基础。 这一目标 应用程序是为了使卡尔森博士能够引导她目前的努力， 老龄化研究：研究职业发展计划由卡尔森博士 承担进行这项研究项目的主要作用，当然， 在科学诚信工作，参加研讨会，期刊俱乐部， 讲习班和科学会议;阅读和讨论原创研究 氧化损伤和动脉粥样硬化的文章;与导师和 在衰老、心血管疾病和细胞凋亡研究方面接受过培训的顾问。 本研究的目的是：1）确定氧化甾醇的具体步骤- 诱导的凋亡途径由改变的ROS生成调节， 培养的血管细胞中的抗氧化剂，和2）表征年龄相关的 加速衰老小鼠（SAM）中动脉粥样硬化病变的发展 模型，并探讨在何种程度上，抗氧化剂补充减少 与年龄相关的动脉粥样硬化病变形成期间的细胞凋亡。 这些 研究将允许候选人测试整体假设， 抗氧化剂在动脉粥样硬化过程中发挥保护作用 通过抑制ROS介导的细胞凋亡伴随衰老。