A muSI-RIAC instrument for multi-bioanalyte assays

描述（由申请人提供）：该资助的目的是开发一种muSI-RIAC仪器，该仪器对微量样品（如尿液、血浆或其他生物基液体）进行循环免疫亲和色谱。该项目将协同联合收割机的上级属性的微量免疫亲和色谱与高精度的能力，微量顺序注射，创造一个非常通用和低成本的仪器平台，用于临床诊断和生物医学分析。 muSI-RIAC仪器将为临床医生和研究人员提供筛选低丰度目标分析物的复杂基质的能力。该设备在许多生物医学领域具有巨大潜力，包括生物技术和药物发现、免疫学和诊断临床分析。该设备将使用微量的样品，并通过多个微亲和柱快速处理，以分离并随后测量目标分子的存在。对公共卫生的益处将影响研究发现和传统临床诊断的进步。 需要对儿科、产后或老年患者进行常规多分析物测定的临床情况可能需要使用常规技术进行过量采样。muSI-RIAC仪器能够用一滴液体（例如血液、脑液、脊髓液或淋巴液）进行所有需要的测定，从而大大降低了患者健康和护理方面的严重不适或危险。 研究发现往往受到需要从高价值但少量复杂样品中进行多组分分析的阻碍。例如，用于药物发现的重组化学、存档血液样品或细胞培养物提取物。筛选低丰度靶分子、分离、定量和检索这些组分的能力将极大地有益于研究界识别和研究重要的生物分析物相互作用的能力，这些相互作用有助于推进其各自的领域。

DESCRIPTION (provided by applicant): The aim of this grant is the development of a muSI-RIAC instrument that performs recycles immunoaffinity chromatography on a micro scale amount of sample such as urine, plasma or other biological based fluids. This project will synergistically combine the superior attributes of micro immunoaffinity chromatography with the high precision capabilities of micro sequential injection to create a very versatile and low cost instrumental platform for clinical diagnostics and biomedical analysis. The muSI-RIAC instrument will provide clinicians and researchers the ability to screen complex matrices for low abundance target analytes. This device holds great potential in many biomedical endeavors including biotechnology and drug discovery, immunology, and diagnostic clinical analysis. This device will use miniscule amounts of sample and quickly process it through multiple micro-affinity columns to isolate and then sequentially measure for the presence of targeted molecules. Benefits to the public health will impact both advancements in Research discovery as well as conventional Clinical diagnostics. Clinical situations where routine multi-analyte assays are required on pediatric, post-natal, or elderly patients may require excess sampling using conventional techniques. The serious discomfort or danger to the patients health and care can be greatly reduced through the muSI-RIAC instrument's ability to perform all needed assays with a single drop of fluid (e.g. blood, cerebral, spinal or lymphatic fluids). Research discovery is often encumbered by the need to perform multiple component analysis from highly valued yet small amounts of complex samples. For example, recombinant chemistries for drug discovery, archival blood samples, or cellular culture extracts. The ability to screen for low abundant target molecules, isolate, quantitative and retrieve such components would highly benefit the research communities ability to identify and study important bio-analyte interactions that serve to advance their respective fields.