Development of Gastrointestinal Transport Models

胃肠道运输模型的发展

• 批准号: 6646290
• 负责人: MARY PAT MOYER
• 金额: $ 37.55万
• 依托单位: INCELL CORPORATION, LLC
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-15 至 2004-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6646290
• 关键词: biological models; cell line; cell population study; clinical research; drug metabolism; gastrointestinal absorption /transport; gastrointestinal drug absorption; gastrointestinal epithelium; human tissue; membrane permeability; model design /development; morphology; tissue /cell culture

DESCRIPTION (provided by applicant): INCELL has successfully completed innovative work establishing several unique human epithelial cell lines of normal small intestine. These cells have been partially characterized and preliminary transport studies suggest that they have characteristics more similar to the small intestine than the Caco-2 cell model. In Phase II, further characterization of these cells will be done. We will examine site-specific differences between the cell lines and determine if passage number or differentiation state effect cellular function. Reproducibility studies of the transport systems will lead to development of a commercial product that can be shipped in a ready to use format. The proposed transport models will be designed to be superior to the current model and serve as the new gold standard for intestinal cell line testing. The addition of these unique transport models, cell cultures and RNA and protein extracts from these cell lines will expand INCELL's products and services capabilities and allow INCELL to capture the majority share of the current markets.

描述（由申请人提供）：INCELL已经成功完成了几种独特的正常小肠人上皮细胞系的创新工作。这些细胞已被部分表征，初步的转运研究表明，它们比Caco-2细胞模型具有更类似于小肠的特征。在第二阶段，将对这些细胞进行进一步的表征。我们将检查细胞系之间的位点特异性差异，并确定传代数或分化状态是否影响细胞功能。运输系统的可重复性研究将导致开发一种商业产品，可以以现成的形式运输。所提出的转运模型将被设计为优于现有模型，并作为肠细胞系检测的新金标准。这些独特的运输模型、细胞培养以及从这些细胞系中提取的RNA和蛋白质将扩大INCELL的产品和服务能力，并使INCELL能够占据当前市场的大部分份额。