Core--Recombinant technologies

核心--重组技术

• 批准号: 6642350
• 负责人: MICHAEL W. QUICK
• 金额: $ 12.63万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6642350
• 关键词: biomedical facility; microinjections; transfection

DESCRIPTION: The Recombinant Technologies Core is envisioned as a source of reagents, equipment, and technical expertise for projects requiring the cellular transfection of DNA and other biologically active molecules. It should be emphasized that this Core is not designed as a service facility, and that experiments are to be performed by the members of the individual Center laboratories under the guidance and supervision of core personnel. The Core is characterized by its four objectives: 1) a vector reservoir for reagents, primarily viral stocks and packaging extracts to be used in transformations; 2) a particle gun supported with hoods and incubators; 3) a small cell injection facility, including an injection apparatus capable of manipulating mouse cells, mouse eggs, drosophila embryos, and cultured neurons; and 4) an oocyte injection facility, designed for the production and injection of frog oocytes. To realize these objectives, the Core will maintain a number of pieces of equipment, including three laminar flow hoods (currently operational); cell culture incubators (three in place, one to be provided by this application); a "gene gun" (already purchased); an inverted fluorescent microscope (to be purchased under this application); and a microinjection station (microscope, pipette puller, manipulator, stable-table, all to be supplied by this application). The Core will be managed on a day-to-day basis by a staff scientist supported by this application; in Years -04 and -05, once the small cell injection facility is operational, the services of a half-time research technician is also requested.

描述：重组技术核心被设想为 需要的项目的试剂、设备和技术专业知识 DNA 和其他生物活性分子的细胞转染。它 应该强调的是，该核心并不是被设计为服务设施，并且 实验将由各个中心的成员进行 实验室在核心人员的指导和监督下。 核心的特点是有四个目标：1）矢量存储库 试剂，主要是病毒原液和包装提取物，用于 转变； 2) 配有防护罩和培养箱的粒子枪； 3）一个 小细胞注射设施，包括能够 操纵小鼠细胞、小鼠卵、果蝇胚胎并培养 神经元； 4) 卵母细胞注射设施，设计用于生产和 注射青蛙卵母细胞。为了实现这些目标，核心将 维护许多设备，包括三个层流罩 （目前正在运营）；细胞培养箱（3个已到位，1个待定） 由本应用程序提供）；一把“基因枪”（已购买）；一个倒立的 荧光显微镜（根据本申请购买）；和一个 显微注射站（显微镜、移液器拉拔器、机械手、稳定工作台、 全部由该应用程序提供）。核心将在 由本应用程序支持的科学家进行日常工作；以年为单位 -04 和 -05，一旦小细胞注入设施投入运行， 还要求提供半职研究技术人员的服务。