Detection of Cortisol using Chromatography and Mass Spectroscopy

使用色谱法和质谱法检测皮质醇

• 批准号: 2111203
• 金额: --
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2111203
• 关键词: Detection; Cortisol; using; Chromatography; Mass

The monitoring of the steroid hormone cortisol/cortisone allows for medical professionals to diagnose conditions often relating to elevated stress and anxiety. Currently the most used method of detecting cortisol requires intravenous sampling and measuring over the course of several hours. Salivary samples have been used to determine the cortisol concentration and hence the hypothalamic pituitary adrenal axis. Mass spectroscopy allows for accurate cortisol detection, using a uSPEed cartridge a chromatographic separation/micro separation allows for improvements to the cortisol signal. Optimising the electrospray micro solid phase extraction (SPE) and atmospheric pressure chemical ionisation (APCI) for different concentrations of cortisol will allow for the development of novel cartridges using silver coated silica tips, with tuneable pores sizes. Additionally, High-Performance Liquid Chromatography (HPLC) with UV detection can be used for detection of cortisol. Coating different papers with silica will allow for lateral flow chromatography, using different sized silicas, and the addition of aptamers means that the paper chromatography can be optimised use in paper spray mass spectroscopy. This will provide a cheap alternative to the current methods of accurately measuring cortisol concentrations.

类固醇激素皮质醇/可的松的监测允许医疗专业人员诊断通常与压力和焦虑升高有关的状况。目前最常用的检测皮质醇的方法需要静脉内采样和测量几个小时。唾液样本已被用来确定皮质醇浓度，因此下丘脑垂体肾上腺轴。质谱允许精确的皮质醇检测，使用uSPEed柱，色谱分离/微量分离允许改善皮质醇信号。优化电喷雾微固相萃取（SPE）和大气压化学电离（APCI）的不同浓度的皮质醇将允许开发新的墨盒使用银涂层的二氧化硅尖端，具有可调的孔径。此外，高效液相色谱法（HPLC）与紫外检测可用于检测皮质醇。用二氧化硅涂覆不同的纸将允许使用不同尺寸的二氧化硅进行侧流色谱，并且适体的添加意味着纸色谱可以优化用于纸喷雾质谱。这将为目前精确测量皮质醇浓度的方法提供一种廉价的替代方法。