CORE-- MICROSCOPE FACILITY

描述：共聚焦和电子显微镜设备配备用于 电子显微镜（EM）和光学显微镜（LM），包括共聚焦和 活细胞成像，包括延时视频和钙成像系统。它 在上一个资助期内， 用于图像处理和分析的成像设备。所有5个项目将使用 基于LM的系统和3将使用EM。除了设备，设施 提供培训和技术支持。该设施由莱斯利博士监督 托尔伯特和雇用三名工作人员：1）硕士水平的高级研究 专家Jansma女士（PPG工作量占60%，部门工作量占40%） 她是EM和共聚焦显微镜的技术专家， 范围，培训新用户，并帮助制定协议。2)兼职 研究技术员，波茨女士（20%的工作在这里，80%的工作在项目2） 提供共聚焦和活细胞系统的培训和帮助。3)一 兼职计算机专家，Yuhas先生（75%的努力在PPG和25%的努力在 司）维修计算机、安装升级软件和培训用户使用软件。

DESCRIPTION: The Confocal and Electron Microscopy Facility is equipped for electron microscopy (EM) and light microscopy (LM) including confocal and live-cell imaging, including time-lapse video and calcium imaging systems. It has also been expanded during the previous funding period to include a Computer Imaging Facility for image processing and analysis. All 5 projects will use the LM-based systems and 3 will use EM. In addition to the equipment, the facility provides training and technical support. The facility is overseen by Dr. Leslie Tolbert and employs three staff members: 1) a Master's level Senior Research Specialist, Ms. Jansma, (60% effort on PPG and 40% for the Division) technically expert in EM and confocal microscopy, she maintains all of the scopes, trains new users and helps to develop protocols. 2) a part-time research technician, Ms. Potts (20% effort here and 80% effort on Project 2) provides training and assistance with the confocal and live-cell systems. 3) a part-time computer expert, Mr. Yuhas (75% effort on PPG and 25% effort in the Division) maintains computers, installs upgrades and trains users in software.