PROPHYLACTIC & THERPEUTIC DNA IMMUNIZATION AGAINST HCV

预防性

• 批准号: 6645482
• 负责人: ALFRED M PRINCE
• 金额: $ 52.72万
• 依托单位: NEW YORK BLOOD CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6645482
• 关键词: Pan; Poxviridae; active immunization; cellular immunity; chimeric proteins; cross immunity; cytokine; cytotoxic T lymphocyte; hepatitis C; hepatitis C virus; humoral immunity; immunogenetics; neutrophil; plasmids; recombinant DNA; recombinant proteins; vaccine development; vector vaccine

The goals of this study are to evaluate the role of humoral and cellular (CTL) effector mechanisms in natural and induced immunity to HCV, and to achieve broadly cross reactive prophylactic immunization. Immune responses will be determined during the development of self-limited or chronic infections in HCV inoculated chimpanzees to evaluate correlates of immunity. The experiments will also evaluate a variety of HCV genomic constructs in plasmids and canary pox vectors, and E1E2 proteins, for induction of humoral and cellular immune responses in chimpanzees. Lymphokine genes (GM-CSF, IL-2 or IL-12) will be used together with HCV viral genes, to assess their efficacy in enhancement of immune responses. The humoral, CD4 T-helper and CTL responses will be analyzed quantitatively and qualitatively. The immunogenicity of HCV genes presented as fusion proteins with ubiquitin and secreting and non-secreting HCV gene-containing plasmids will be compared.

本研究的目的是评估体液和细胞（CTL）效应机制在HCV自然和诱导免疫中的作用，并实现广泛的交叉反应性预防性免疫。免疫反应将在丙型肝炎疫苗接种黑猩猩的自限性或慢性感染发展过程中确定，以评估免疫的相关因素。这些实验还将评估质粒和金丝雀痘载体中的各种HCV基因组结构，以及E1E2蛋白，以诱导黑猩猩的体液和细胞免疫反应。淋巴因子基因（GM-CSF、IL-2或IL-12）将与丙型肝炎病毒基因一起使用，以评估它们在增强免疫反应方面的功效。将定量和定性地分析体液、CD4 t辅助细胞和CTL反应。将比较HCV基因与泛素融合蛋白、含HCV基因的分泌质粒和非分泌质粒的免疫原性。

项目成果

Immunoprophylaxis of hepatitis C virus infection.

丙型肝炎病毒感染的免疫预防。

• DOI: 10.1016/s1089-3261(05)70211-7
• 发表时间: 2001
• 期刊: Clinics in liver disease.
• 作者: Prince,AM;Shata,MT
• 通讯作者: Shata,MT

gC1qR expression in chimpanzees with resolved and chronic infection: potential role of HCV core/gC1qR-mediated T cell suppression in the outcome of HCV infection.

• DOI: 10.1016/j.virol.2005.11.020
• 发表时间: 2006-03
• 期刊: Virology
• 影响因子: 3.7
• 作者: Z. Yao;M. T. Shata;Nancy Tricoche;M. Shan;B. Brotman;W. Pfahler;Y. Hahn;A. Prince

Optimization of recombinant vaccinia-based ELISPOT assay.

基于重组牛痘的 ELISPOT 测定的优化。

• DOI: 10.1016/j.jim.2003.10.002
• 发表时间: 2003
• 期刊: Journal of immunological methods
• 影响因子: 2.2
• 作者: Shata,MohamedTarek;Shan,MeiMei;Tricoche,Nancy;Talal,Andrew;Perkus,Marion;Prince,Alfred
• 通讯作者: Prince,Alfred

Automation of nucleic acid extraction for NAT screening of individual blood units.

自动化核酸提取，用于单个血液单位的 NAT 筛查。

• DOI: 10.1046/j.1537-2995.2001.41040483.x
• 发表时间: 2001
• 期刊: Transfusion
• 影响因子: 2.9
• 作者: Lee,DH;Prince,AM
• 通讯作者: Prince,AM

Attempted therapeutic immunization in a chimpanzee chronic HBV carrier with a high viral load.

尝试对高病毒载量的黑猩猩慢性乙型肝炎病毒携带者进行治疗性免疫。

• DOI: 10.1111/j.1600-0684.2006.00152.x
• 发表时间: 2006
• 期刊: Journal of medical primatology
• 影响因子: 0.7
• 作者: Shata,MohamedTarekM;Pfahler,Wolfram;Brotman,Betsy;Lee,Dong-Hun;Tricoche,Nancy;Murthy,Krishna;Prince,AlfredM
• 通讯作者: Prince,AlfredM