CORE--MOLECULAR BIOLOGY LABORATORY

核心--分子生物学实验室

• 批准号: 6580341
• 负责人: JAMES K. MCDOUGALL
• 金额: $ 28.07万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580341
• 关键词: biomedical facility; human papillomavirus; molecular biology; nucleic acid hybridization; polymerase chain reaction; reproductive system neoplasm; viral carcinogenesis; virus DNA; virus classification

The Molecular Biology Laboratory (Core Component A) will analyze sections from archival paraffin blocks of tissue from cases and controls in the study group of Project 1. The selection of tissues to be tested will be made in the Pathology Core Component B, which will be responsible for the receipt, tracking and return of tissues blocks. All specimens will be tested for the presence of human papillomavirus DNA using the polymerase chain reaction (PCR) amplification method. This core provides a centralized test procedure for application to all studies.

分子生物学实验室（核心组件A）将分析项目1研究组中病例和对照的存档石蜡组织块切片。待测组织的选择将在病理学核心组件B中进行，该组件将负责组织块的接收、跟踪和返回。将使用聚合酶链反应（PCR）扩增方法检测所有标本是否存在人乳头瘤病毒DNA。该核心提供了适用于所有研究的集中检测程序。