CORE--IMMUNOELECTRON MICROSCOPY

免疫细胞化学已成为细胞生物学研究的有力工具。 它允许对单个蛋白质进行精确的细胞定位 细胞器和--当被带到电子显微镜水平上--甚至 其特定小舱。它提供了有关本地化的信息 在完整细胞中的蛋白质，因此是必不可少的，因为它 补充细胞分级和生化方法。申请 在电子显微镜水平上的免疫细胞化学方法在技术上 要求苛刻，需要广泛的设备和高度熟练的 经验丰富的人员。核心B的目的是提供此专业知识 并使计划项目的所有成员都可以应用这些 技术在他们的项目相关研究中。与以下项目关联的人员 核心在目前使用的所有主要方法方面都有相当丰富的经验 在涉及免疫荧光、免疫过氧化物酶的免疫细胞化学中 技术和超薄冰冻切片的免疫金标记法。《核心》 主任在她的研究中使用了这些技术超过15年。 麦卡弗里，核心的技术总监，已经有超过15年的时间 在这一领域的经验，并与人合著了许多出版物， 包括在本供资期间使用这些技术的10个。 在过去的四年里，在计划项目的赞助下，我们 已将该设施作为一流的核心服务计划进行运营 会员。核心已经被所有项目负责人大量使用，这是可以证明的 25家出版物利用了核心设施和/或 人事部。核心专业知识的可获得性 提供了新的信息，导致了新的研究方向 项目负责人。

Immunocytochemistry has been a powerful tool in research in cell biology. It allows the precise localization of individual proteins to cell organelles and--when carried to the electron microscope level--even to specific subcompartments thereof. It provides information on localization of proteins in intact cells and therefore is essential in that it complements cells fractionation and biochemical approaches. To apply immunocytochemical methods at the electron microscope level is technically demanding and requires extensive instrumentation and highly skilled and experienced personnel. The purpose of Core B is to provide this expertise and make it possible for all member of the Program Project to apply these techniques in their program-related research. Personnel associated with the Core have considerable experience in all major approaches current used in immunocytochemistry involving immunofluorescence, immunoperoxidase techniques, and immunogold labeling of ultrathin cryosections. The Core Director has used these techniques in her research over 15 years, and Mr. McCaffery, the Core's Technical Director, has had over 15 years experiences in this area and has co-authored a number of publications, including 10 during the current funding period using these techniques. During the last four years under the auspices of the Program Project, we have operated the facility as a state-of-the-ar Core to serve program members. The Core has been heavily used by all Project leaders as attested to by the fact that 25 publications have utilized core facilities and/or personnel. Availability of the expertise of the expertise of the core has provided new information that led to new research direction for the Project Leaders.