Confocal Core Facility for Vision Researchers

视觉研究人员的共焦核心设施

• 批准号: 6653653
• 负责人: A. Sue Menko
• 金额: $ 57.51万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653653
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; biomedical facility; confocal scanning microscopy; fluorescence microscopy; health science research; vision

DESCRIPTION (provided by applicant): This R24 Grant is submitted to establish a state-of-the-art Confocal Laser Scanning Microscope Core Facility for vision researchers at Thomas Jefferson University (TJU). It will provide a well-established group of NEI-funded vision researchers at TJU the means to perform cutting edge fluorescence microscopy analysis of both fixed and live cells and tissues, greatly enhancing their vision research programs. This facility will also serve collaborators and colleagues of the NEI-funded investigators who have an interest in vision biology. It is hoped that the advanced technology offered by this facility will attract additional investigators at TJU to apply their knowledge to questions in vision research, either independently or in collaboration with the core group of investigators. The confocal microscope we propose to purchase, the Zeiss LSM 510 META, well addresses the needs of the vision investigators at TJU to examine the complex and dynamic relationships between proteins at the cellular, subcellular and molecular level. It offers the unique capability of emission fingerprinting which can separate up to 8 different fluorescent markers despite overlap of their emission spectra, thereby permitting analysis of the molecular relationships of distinct cellular components. This microscope makes it simple to perform dynamic FRAP, FLIP and FRET analyses. It can resolve rapid cellular processes and allows for quantitative analysis of image series. It is expected that this confocal facility not only will enhance the current research goals of this group of vision researchers but also provide new avenues for their research projects not before possible. Access to this confocal microscope should allow this dynamic group of vision researchers, who have already provided many important contributions to their specific fields, to both enhance and expand their research directions and to increase their productivity, without technology being a limitation. It is our long term goal, as we reach the required nucleus of NEI-funded investigators, to expand this facility into a larger Core Facility for vision researchers at TJU and Wills Eye Hospital. We envision this core as providing not only additional technologies that will enhance the research activity of these investigators, but also to provide a central site that will foster intellectual interactions between the investigators and lead to even greater cooperation and collaboration within the TJU vision community.

描述（由申请人提供）：此R24赠款提交建立一个国家的最先进的共聚焦激光扫描显微镜核心设施的视觉研究人员在托马斯杰斐逊大学（TJU）。它将为TJU的NEI资助的视觉研究人员提供一个完善的团队，对固定和活细胞和组织进行尖端荧光显微镜分析，大大增强他们的视觉研究计划。该设施还将为NEI资助的研究人员的合作者和同事提供服务，他们对视觉生物学感兴趣。希望该设施提供的先进技术将吸引更多的研究人员在TJU应用他们的知识在视觉研究的问题，无论是独立或与核心组的研究人员合作。我们建议购买的共聚焦显微镜，蔡司LSM 510 Meta，很好地解决了TJU视觉研究人员的需求，以检查蛋白质在细胞，亚细胞和分子水平上的复杂和动态关系。它提供了独特的发射指纹识别能力，可以分离多达8种不同的荧光标记物，尽管它们的发射光谱重叠，从而允许分析不同细胞组分的分子关系。这种显微镜可以简单地进行动态FRAP，FLIP和FRET分析。它可以解决快速的细胞过程，并允许图像系列的定量分析。预期此共焦装置不仅将提升视觉研究者目前的研究目标，也将为他们的研究项目提供前所未有的新途径。访问这个共聚焦显微镜应该允许这个动态组的视觉研究人员，谁已经提供了许多重要的贡献，他们的特定领域，以加强和扩大他们的研究方向，并提高他们的生产力，没有技术是一个限制。这是我们的长期目标，因为我们达到了NEI资助的研究人员所需的核心，扩大这个设施成为一个更大的核心设施，为视觉研究人员在TJU和威尔斯眼科医院。我们设想这个核心不仅提供额外的技术来增强这些研究人员的研究活动，而且还提供一个中心站点，以促进研究人员之间的智力互动，并在TJU视觉社区内带来更大的合作与协作。