Confocal Core Facility for Vision Researchers

视觉研究人员的共焦核心设施

• 批准号: 6653653
• 负责人: A. Sue Menko
• 金额: $ 57.51万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653653
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; biomedical facility; confocal scanning microscopy; fluorescence microscopy; health science research; vision

DESCRIPTION (provided by applicant): This R24 Grant is submitted to establish a state-of-the-art Confocal Laser Scanning Microscope Core Facility for vision researchers at Thomas Jefferson University (TJU). It will provide a well-established group of NEI-funded vision researchers at TJU the means to perform cutting edge fluorescence microscopy analysis of both fixed and live cells and tissues, greatly enhancing their vision research programs. This facility will also serve collaborators and colleagues of the NEI-funded investigators who have an interest in vision biology. It is hoped that the advanced technology offered by this facility will attract additional investigators at TJU to apply their knowledge to questions in vision research, either independently or in collaboration with the core group of investigators. The confocal microscope we propose to purchase, the Zeiss LSM 510 META, well addresses the needs of the vision investigators at TJU to examine the complex and dynamic relationships between proteins at the cellular, subcellular and molecular level. It offers the unique capability of emission fingerprinting which can separate up to 8 different fluorescent markers despite overlap of their emission spectra, thereby permitting analysis of the molecular relationships of distinct cellular components. This microscope makes it simple to perform dynamic FRAP, FLIP and FRET analyses. It can resolve rapid cellular processes and allows for quantitative analysis of image series. It is expected that this confocal facility not only will enhance the current research goals of this group of vision researchers but also provide new avenues for their research projects not before possible. Access to this confocal microscope should allow this dynamic group of vision researchers, who have already provided many important contributions to their specific fields, to both enhance and expand their research directions and to increase their productivity, without technology being a limitation. It is our long term goal, as we reach the required nucleus of NEI-funded investigators, to expand this facility into a larger Core Facility for vision researchers at TJU and Wills Eye Hospital. We envision this core as providing not only additional technologies that will enhance the research activity of these investigators, but also to provide a central site that will foster intellectual interactions between the investigators and lead to even greater cooperation and collaboration within the TJU vision community.

描述(由申请者提供)：这份R24赠款是为托马斯·杰斐逊大学(TJU)的视觉研究人员建立一个最先进的共焦激光扫描显微镜核心设施。它将为天津大学NEI资助的视力研究人员提供对固定和活细胞和组织进行尖端荧光显微镜分析的手段，极大地增强他们的视力研究计划。该设施还将为NEI资助的研究人员的合作者和同事提供服务，他们对视觉生物学感兴趣。希望这一设施提供的先进技术将吸引更多的TJU调查人员将他们的知识应用于视觉研究中的问题，无论是独立的还是与核心调查人员小组合作。我们计划购买的蔡司LSM 510META共聚焦显微镜很好地满足了TJU视觉研究人员在细胞、亚细胞和分子水平上研究蛋白质之间复杂和动态关系的需求。它提供了独特的发射指纹识别能力，即使发射光谱重叠，也可以分离多达8种不同的荧光标记，从而能够分析不同细胞成分的分子关系。该显微镜使执行动态FRAP、FLIP和FRET分析变得简单。它可以分解快速的细胞过程，并允许对图像序列进行定量分析。预计这一共焦设施不仅将提高这一组视觉研究人员目前的研究目标，而且还将为他们的研究项目提供新的途径。接触到这种共焦显微镜应该会让这群充满活力的视觉研究人员，他们已经在各自的特定领域做出了许多重要贡献，既可以加强和扩大他们的研究方向，也可以提高他们的生产率，而不是技术的限制。这是我们的长期目标，随着我们达到NEI资助的研究人员所需的核心，将该设施扩展为TJU和Wills眼科医院的视觉研究人员的更大核心设施。我们设想这一核心不仅将提供额外的技术来加强这些调查人员的研究活动，而且还将提供一个中央站点，促进调查人员之间的智力互动，并在TJU VISION社区内带来更大的合作和协作。