TISSUE ENGINEERING FOR PERIODONTIUM REPAIR

描述：(逐字)组织工程学的定义是科学发展 新组织的制造技术用于替代和再生 根据细胞和发育生物学原理丢失的组织。其中一个 老龄化、牙周病、牙种植体、移植、 或再植仍然是牙周组织的修复和再生。 牙周组织，如牙骨质、牙周膜和牙槽骨。 一般来说，要实现完全修复和再生，有必要 概述了胚胎发生和形态发生过程中涉及的 这种组织的形成。如果是牙周组织，则为完整的牙周 修复需要牙骨质形成、成骨和牙周韧带修复。 纤维。目前牙周修复的策略针对的是 促进牙槽骨和牙周韧带再生，并具有 不能完全恢复牙周组织结构的问题。 这个项目的长期目标是实现完整的牙周组织 通过组织工程学聚焦牙骨质层进行再生 诱导牙骨质形成。我们建议检验一下她的假设 体外培养的细胞可以重新定植牙骨质剥离的根部并重新生成 牙骨质形成的过程。此外，类牙骨质矿化 这些细胞在体外沉积的细胞外基质(或其部分 成分)会导致牙周的完全再生。我们打算测试一下 这一假设是通过使用永生小鼠衍生的HERS细胞系建立的 我们的实验室在活体内重新定植根牙本质。在类似的实验中， 由这些细胞产生的细胞外基质和一些分离的 成分(主要是釉质蛋白)也将被测试其诱导和 修复能力。然后，这项研究中获得的结果可以应用于 单独或与已知的骨再生技术结合使用，用于 治疗牙周病，提高远期疗效 植入物。这项研究中产生的数据也将提供信息 与根形成的调节有关，以及改善根形成的可能途径 因年龄或牙周原因导致牙齿缺失的诊断、治疗和预后 疾病。

DESCRIPTION: (verbatim) Tissue Engineering is defined as the science developing techniques for fabrication of new tissues for replacement and regeneration of lost tissues based on principles of cell and developmental biology. One of the major problems in aging, periodontal disease, tooth implants, transplantation, or replantation remains the repair of the periodontium and the regeneration of periodontal tissues such as cementum, periodontal ligament and alveolar bone. In general, to achieve complete repair and regeneration, it is necessary to recapitulate the process of embryogenesis and morphogenesis involved in the formation of that tissue. In the case of the periodontium, complete periodontal repair entails de novo cementogenesis, osteogenesis, and periodontal ligament fibers. Current strategies for periodontal repair have been directed at enhancing alveolar bone and periodontal ligament regeneration and have the problem that do not completely restore the architecture of the periodontium. The long-term objective of this project is to achieve complete periodontium regeneration by focusing on the cementum layer using tissue engineering to induce de novo cementogenesis. We propose to test the hypothesis that HERS cells maintained in vitro can re-colonize cementum denuded roots and recreate the process of cementogenesis. Furthermore, the cementum-like mineralized extracellular matrix deposited by these cells in vitro (or some of its components) will induce complete periodontal regeneration. We propose to test this hypothesis by using an Immortomouse-derived HERS cell line established in our laboratory to re-colonize root dentine in vivo. In similar experiments, the extracellular matrix produced by these cells and some of its isolated components (mainly enamel proteins) will also be tested for their inductive and reparative capability. The results obtained in this study can then be applied, alone or in conjunction with known techniques for bone regeneration, for the treatment of periodontal disease and to improve the long-term success of implants. The data generated in this study will also provide information related to the regulation of root formation and possible ways to improve the diagnosis, treatment, and prognosis of tooth loss due to aging or periodontal diseases.