CYTOTOXIC T LYMPHOCYTE ACTIVATION IN VITRO AND IN VIVO

体外和体内细胞毒性 T 淋巴细胞激活

• 批准号: 6624630
• 负责人: MATTHEW Franklin MESCHER
• 金额: $ 21.58万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 2003-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6624630
• 关键词: MHC class I antigen; T cell receptor; artificial membranes; cytokine; cytokine receptors; cytotoxic T lymphocyte; genetically modified animals; immunologic memory; interleukin 12; interleukin 2; laboratory mouse; leukocyte activation /transformation; passive immunization; receptor expression; tissue /cell culture

DESCRIPTION: (Adapted from the applicant's abstract) In this application, Dr. Mescher proposes to continue his studies identifying ligands that are essential for the activation of naive and memory cytotoxic T lymphocytes. Utilizing both his pioneering system of artificial surfaces for the delivery of defined signals and an adoptive transfer of CTLs from transgenic TCR mice, Dr. Mescher proposes to confirm much of his preliminary work identifying the key signals responsible for triggering CTL activation. This renewal application is expanded to include experiments to define the potential role that IL-12 plays in activating the naive CTL response as well as investigating a potential role for inflammatory cytokines in influencing memory cell function. Two specific aims, which are essentially identical to the specific aims of the original project, with the important addition of the adoptive transgenic mouse system are proposed.

描述：（改编自申请人的摘要）在此 应用程序，Mescher博士建议继续他的研究，确定 激活幼稚和记忆所必需的配体 细胞毒性T淋巴细胞利用他的开创性系统， 用于传递定义的信号的人工表面，以及 从转基因TCR小鼠中转移CTL，Mescher博士建议 证实了他的大部分初步工作， 负责触发CTL激活。此更新申请是 扩大到包括实验，以确定IL-12的潜在作用， 在激活幼稚CTL反应以及研究 炎性细胞因子在影响记忆细胞中的作用 功能两个具体的目标，基本上是相同的， 原始项目的具体目标，重要的是增加了 提出了过继转基因小鼠系统。