Output of the Circadian Clock

描述(申请人提供)：昼夜节律(-24小时) 由生物钟控制，在许多人的生理和行为中都发现了 包括人类和果蝇在内的有机体。在哺乳动物中，睡眠-醒来周期是 昼夜节律可以产生重要影响的最明显的例子 但对体温、心跳、血压的调节， 内分泌功能、肾脏活动和肝脏代谢也被显示出来。 处于昼夜节律之下。生物钟产生振荡 控制时钟本身节奏的转录模式 以及它们所产生的产量。这项研究项目的目标是 第一个在全基因组范围内转录成人头的测试 果蝇受生物钟调节，然后识别出 这些参与调节静息活动的转录本 周而复始。将使用DNA微阵列进行表达分析； 运动活动分析将作为一种表型测量 休息-活动循环；基因和分子技术的结合将 被用于对候选调节器的功能分析 休息-活动循环。钟控器的综合描述 果蝇头部的转录将为所有未来提供宝贵的资源 对昼夜节律的研究，而发现新的 休息-活动循环将促进对分子的更好理解 水果文件中休息-活动循环的基础机制和 哺乳动物中类似的睡眠-觉醒周期。

DESCRIPTION (provided by applicant): Circadian (-24 hour) rhythms that are controlled by circadian clocks are found in the physiology and behavior of many organisms including humans and fruitflies. In mammals the sleep-wake cycle is the most obvious example of a circadian rhythm that can have important effects on health, but the regulation of body temperature, heartbeat, blood pressure, endocrine functions, renal activity, and liver metabolism have also been shown to be under circadian regulation. Circadian clocks generate oscillating patterns of transcription that control both the rhythm of the clocks themselves and the output that they produce. It is the goal of this research project to first test on a genome-wide scale which transcripts in the head of adult fruitflies are regulated by circadian clocks and then identify a subset of these transcripts that are involved in the regulation of the rest-activity cycle. DNA microarrays will be used to perform the expression analyses; locomotor activity assays will serve as a phenotypic measure for the rest-activity cycle; and a combination of genetic and molecular techniques will be employed in the functional analysis of candidate regulators of the rest-activity cycle. The comprehensive description of the clock controlled transcripts in Drosophila heads will provide a valuable resource for all future studies on circadian rhythms, whereas the discovery of new mediators of the rest-activity cycle will promote a better understanding of the molecular mechanisms underlying both the rest-activity cycle in fruitfiles and the analogous sleep-wake cycle in mammals.