DIFFRACTION ANALYSIS OF FLP RECOMBINASE & ANIMAL LECTINS

FLP 重组酶的衍射分析

• 批准号: 6667819
• 负责人: JAMES RINI
• 金额: $ 14.27万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2003-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6667819
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; proteins; radiography; structural biology

Our interest is in the catalytic mechanism of type I DNA topoisomerases, in particular E. coli DNA topoisomerases I and III. We have been studying the interactions of topoisomerase I with DNA. We have collected several data sets from crystals either soaked or co-crystallized in the presence of different oligo- and mono-nucleotides. We have also collected high resolution data on a fragment of topoisomerase I that forms the top part of the molecule. We are interested in the changes that occur in the molecule during different stages of the reaction and this fragment may illustrate the open conformation of the molecule. We also collected some preliminary data on crystals of the RNA component of B. subtilis RNase P. The crystals diffract to 4A resolution at CHESS.

我们感兴趣的是I型dna的催化机制。 拓扑异构酶，特别是大肠杆菌DNA拓扑异构酶I和III。 我们一直在研究拓扑异构酶I与DNA的相互作用。 我们收集了几组来自浸泡过的晶体或 在不同的齐聚物存在下共结晶 单核苷酸。我们还收集了高分辨率的数据 拓扑异构酶I的片段，构成分子的顶部。 我们对分子在过程中发生的变化很感兴趣 反应的不同阶段，这一片段可以说明 分子的开放构象。我们还收集了一些初步的 枯草杆菌核糖核酸酶核糖核酸组分晶体的研究 在国际象棋中，晶体的分辨率可达4A。