Tumor Host Range Mutants of Polyoma and Their Targets

多瘤的肿瘤宿主范围突变体及其靶标

• 批准号: 6649274
• 负责人: THOMAS Livingston BENJAMIN
• 金额: $ 57.46万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6649274
• 关键词: DNA replication; Polyomavirus muris 1; host neoplasm interaction; host organism interaction; mutant; neoplasm /cancer genetics; ovary neoplasms; tissue /cell culture; tumor suppressor genes; viral carcinogenesis; virus DNA; virus antigen; virus genetics; virus infection mechanism; virus related neoplasm /cancer; virus replication; yeast two hybrid system

DESCRIPTION (provided by the applicant): This project concerns the use of a 'tumor host range' (T-HR) selection procedure for isolation of host range mutants of Polyoma virus. The procedure involves screening and selection of virus mutants for their ability to grow on non-polyoma transformed or tumor-derived cells and inability to grow in normal primary mouse cells. The procedure is coupled to the yeast two hybrid system using wild type virus T antigen sequences as bait to screen mouse embryo cDNA libraries. Absence of interaction of target cDNAs with the mutant bait is taken as an indication of a physiologically relevant target in virus replication or transformation. The procedure is viewed as a possible way to uncover new tumor suppressor genes or other factors with which the virus must interact in normal cells in order to grow and which are altered or missing in cancer cells. We will use results from a partially characterized T-HR mutant and its putative tumor suppressor gene target to produce a mouse model of ovarian carcinoma.

描述(由申请人提供)：本项目涉及使用 肿瘤宿主范围(T-HR)选择程序用于分离宿主范围 多瘤病毒的突变体。该程序包括筛选和选择 病毒突变体在非多瘤转化的肿瘤上生长的能力 肿瘤来源的细胞和不能在正常的原代小鼠细胞中生长。这个 程序与使用野生型病毒T的酵母双杂交系统偶联 以抗原序列为诱饵筛选小鼠胚胎c DNA文库。缺席 靶DNA与突变诱饵的相互作用被认为是一种 病毒复制或转化中与生理相关的靶点。这个 手术被认为是发现新的肿瘤抑制基因或 病毒在正常细胞中必须与之相互作用的其他因素 在癌细胞中生长并被改变或缺失的基因。我们将使用以下结果 一个部分特征的T-HR突变体及其可能的肿瘤抑制基因 目的：制作一种小鼠卵巢癌模型。