Modelling, design and evaluation of a new in-situ, real time, affinity-based biosensor for monitoring release of stress markers from Chinese Hamster

一种新型原位、实时、基于亲和力的生物传感器的建模、设计和评估，用于监测中国仓鼠应激标记物的释放

• 批准号: 2126621
• 金额: --
• 依托单位: University of the West of England
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2126621
• 关键词: Modelling; design; evaluation; new; situ

Currently, online monitoring used to optimise protein synthesis, involving either measurement of cell mass or environmental parameters, fail to provide information relating to the physiological integrity of the cells. In-situ monitoring of cell-quality markers has the advantage that changes will allow critical phenomena, such as physiological deterioration of the cell, to be predicted.This project aims to investigate a new bio-sensing modality for real-time monitoring of intracellular biomarkers, as indicators of cell health within a bioreactor. It will involve concurrent, integrated development of a nanocrystalline, porous 3D biosensor, with robust, biocompatible encapsulation, and quantify the impact of bioreactor inputs on these measured cell-quality parameters.The longer term goal is to arrive at a deeper understanding of the biological processes that exist within a bioreactor, and thereby enhance quality control and reliability in bio-manufacturing.

目前，用于优化蛋白质合成的在线监测，包括测量细胞质量或环境参数，无法提供与细胞生理完整性相关的信息。对细胞质量标记物进行原位监测的优点是，这些变化将允许预测关键现象，例如细胞的生理退化。该项目旨在研究一种新的生物传感方式，用于实时监测细胞内生物标志物，作为生物反应器内细胞健康的指标。它将涉及同步，集成开发纳米晶体，多孔3D生物传感器，具有强大的，生物相容的封装，并量化生物反应器输入对这些测量细胞质量参数的影响。长期目标是对生物反应器内存在的生物过程有更深入的了解，从而提高生物制造的质量控制和可靠性。