IDBEST Differential Display on Protein Chips

描述（由申请人提供）：DNA和蛋白质芯片的差异显示分析已被证明在癌症生物标志物的鉴定中很有用。同位素标记方法，如ICAT，有可能显著提高这些方法的精度，但目前不能应用于蛋白质芯片，因为标记的肽不能有效地与未标记的肽分离。然而，通过将原子序数在35 （Br）和63 （Eu）之间的一种元素加入到同位素标签中，所得到的标记肽在质谱中移动了0.1 amu。正如我们的初步工作所表明的那样，这些同位素分化的结合能转移标签（IDBEST）允许在质谱中直接检测未标记物种的标记肽，而无需亲和或液相色谱清理样品。该项目的目标是开发同位素差异化结合能转移标签（IDBEST）和相关的质谱滤波算法，从而可以在亲和蛋白芯片上直接进行高精度（<10%标准偏差）差分显示。

DESCRIPTION (provided by applicant): Differential display analysis on DNA and protein chips has proven useful in the identification of biomarkers for cancer. Isotope tagging methods, such as ICAT, have the potential to significantly improve the precision of these methods, but cannot currently be applied to protein chips where the tagged peptides cannot be effectively separated from untagged peptides. However, by incorporating one of the elements with atomic numbers between 35 (Br) and 63 (Eu) into isotopic tags, the resulting tagged peptides are shifted in the mass spectrum by 0.1 amu. As our preliminary work shows, these isotope differentiated binding energy shift tags (IDBEST) allow direct detection of the tagged peptides from untagged species in the mass spectrum without the need for affinity or liquid chromatography clean up of the sample. The goal of this project is to develop isotope differentiated binding energy shift tags (IDBEST) and associated mass spectral filtering algorithms that allow high precision (<10% standard deviation) differential display to be conducted directly on affinity protein chips.