Multiplexed Analysis of Protein Tyrosine Kinases

蛋白质酪氨酸激酶的多重分析

• 批准号: 6584773
• 负责人: Qing X Yang
• 金额: $ 16.28万
• 依托单位: SUPERARRAY BIOSCIENCE CORPORATION
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-20 至 2004-02-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6584773
• 关键词: 3T3 cells; antibody; biological signal transduction; charge coupled device camera; enzyme linked immunosorbent assay; microarray technology; neoplastic process; nucleic acid hybridization; nucleic acid sequence; oligonucleotides; polymerase chain reaction; protein protein interaction; protein tyrosine kinase; technology /technique development; transfection

DESCRIPTION (provided by applicant): The long-term goal of this proposal is to develop an advance tool that allows simultaneous and ultrasensitive analysis of multiple protein kinase antigens. Such platform is urgently needed in cancer research and clinical laboratories. Multiplex analysis of protein tyrosine kinases (PTKs) expressing or co-expressing in many of the cancer cells such as breast cancers will certainly provide much more straightforward tool for cancer research as well as drug discovery. The ID-Tag Protein Array (IDPA) technology developed in SuperArray Inc. can be used for the PTK multiplexed assays. This technology is based on design of diverse and unique ID-Tags to convert the protein-protein interaction signals in suspension array to be able to be detected and decoded on a DNA array by the advance technology developed in SuperArray Inc. for DNA microarrays.

描述（由申请人提供）：该提案的长期目标是开发一种先进的工具，允许同时和超灵敏地分析多种蛋白激酶抗原。癌症研究和临床实验室迫切需要这样的平台。蛋白酪氨酸激酶（Protein Tyrosine Kinases，PTKs）在乳腺癌等多种肿瘤细胞中的表达和共表达的多重分析，无疑将为肿瘤研究和药物开发提供更为直接的工具。SuperArray Inc.开发的ID-Tag蛋白质阵列（IDPA）技术。可用于PTK多重测定。该技术基于设计多样且独特的ID-Tags，以将悬浮阵列中的蛋白质-蛋白质相互作用信号转换为能够通过SuperArray Inc.开发的先进技术在DNA阵列上检测和解码。用于DNA微阵列。