Higher throughput comet assay for genotoxicity studies

用于基因毒性研究的更高通量彗星测定

• 批准号: 6583673
• 负责人: JANOS LUKA
• 金额: $ 10万
• 依托单位: TREVIGEN, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-15 至 2004-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6583673
• 关键词: DNA damage; adduct; biotechnology; cell population study; gel electrophoresis; high throughput technology; image processing; method development; statistics /biometry; toxicant screening

DESCRIPTION (provided by applicant): The comet assay allows for the detection of DNA damage in any accessible cell population and a molecular definition of the DNA damage is possible with adaptation of the basic assay. Trevigen has developed a platform for the comet assay consisting of a specially treated glass support, promoting the adherence of agarose, with demarcated sample application areas. This phase I proposal describes the development and validation of a higher-throughput format for comet assay. The system will consist of a support with multisample application areas pretreated for direct sample application for use with standard microscope platforms and current data analysis packages. The prototype will be validated for both the standard and the modified comet assay. The long term objective is to provide a rapid, cheap, standardized, and validated assay for the monitoring of genotoxicity of drugs or other treatments. The assay can be applied to both cells in culture for assessment of drugs very early in the screening process, and to samples obtained from animal tissue for specific organ toxicity monitoring of drug metabolites.

描述（由申请方提供）：彗星试验允许检测任何可接近的细胞群中的DNA损伤，并且通过调整基本试验可以对DNA损伤进行分子定义。Trevigen开发了一种彗星试验平台，该平台由经过特殊处理的玻璃支持物组成，可促进琼脂糖的粘附，并具有划定的样品应用区域。本阶段I提案描述了彗星试验的高通量格式的开发和验证。该系统将包括一个支持多样品应用领域的预处理直接样品应用与标准显微镜平台和当前的数据分析包使用。将对标准和改良彗星试验的原型进行验证。长期目标是提供一种快速、廉价、标准化和有效的检测方法，用于监测药物或其他治疗的遗传毒性。该测定法可应用于培养物中的细胞以在筛选过程的非常早期评估药物，并且可应用于从动物组织获得的样品以用于药物代谢物的特定器官毒性监测。