Chemical Tools for Regulating Eukaryotic Transcription
调节真核转录的化学工具
基本信息
- 批准号:6798076
- 负责人:
- 金额:$ 4万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-04-01 至 2007-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION: (provided by applicant) The sequencing of the human genome coupled
with continued advances in proteomics and genomics have led to an unprecedented
understanding of the relationship between genetic content and disease. As a
consequence, the development of transcription-based therapeutics that would
selectively reprogram aberrant gene expression in diseased cells is an
increasingly attractive goal. There remain, however, fundamental questions
surrounding the regulatory mechanism of transcription as well as the
requirements for specificity and activity of such non-natural transcription
factors. Many of these questions have proven intractable by genetic or
biochemical means alone. Described herein is an approach for the generation of
unique chemical tools that will be used for addressing basic questions of
transcriptional regulation. These tools will further serve as molecular targets
for the long-term development of transcription-based therapeutics.
Saccharomyces cerevisiae or budding yeast will serve as our model system due to
the mechanistic homology with mammalian systems, the availability of genomic
information, and because yeast-specific transcriptional activators and/or
repressors will serve as excellent candidates for anti-fungal agents.
In order to accomplish these goals, transcriptional regulators will be selected
from libraries of peptides screened for binding to the Mediator complex
component Gall 1. A battery of biochemical techniques will be used to
characterize the binding affinity and specificity of all ligands thus selected.
In vitro transcription experiments will be employed to functionally
characterize the ligands as activators or inhibitors of transcription that will
be used to probe mechanistic questions surrounding transcriptional regulation
in experiments designed to map functionally important protein-protein
interactions and to probe the origin of synergy in transcriptional activation.
As Saccharomyces cerevisiae is an excellent model system for metazoans, the
data thus obtained will significantly contribute to the evolving model of
eukaryotic transcriptional activation. Furthermore, the chemical regulators
developed throughout the project will serve as the basis for achievement of the
long-term goal of the design and investigation of transcription-based
therapeutics. The protein under investigation, Galil, is a yeast-specific
component of the transcriptional apparatus; thus artificial regulators that
exert their function via specific interactions with Gall 1 will function only
in yeast, providing a mechanism for the development of anti-fungal agents.
描述:(由申请人提供)人类基因组偶联测序
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('ANNA K. MAPP', 18)}}的其他基金
Probing transcriptional activation at the molecular level
在分子水平上探测转录激活
- 批准号:
10370326 - 财政年份:2020
- 资助金额:
$ 4万 - 项目类别:
Probing transcriptional activation at the molecular level - Equipment Supplement
在分子水平上探测转录激活 - 设备补充
- 批准号:
10604581 - 财政年份:2020
- 资助金额:
$ 4万 - 项目类别:
Probing transcriptional activation at the molecular level
在分子水平上探测转录激活
- 批准号:
10594975 - 财政年份:2020
- 资助金额:
$ 4万 - 项目类别:
Probing Transcriptional Activation at the Molecular Level
在分子水平上探测转录激活
- 批准号:
8111363 - 财政年份:2010
- 资助金额:
$ 4万 - 项目类别:
Small Molecule Inhibitors of the Transcriptional Activators ESX and NF-kB
转录激活剂 ESX 和 NF-kB 的小分子抑制剂
- 批准号:
8223254 - 财政年份:2009
- 资助金额:
$ 4万 - 项目类别:
Small Molecule Inhibitors of the Transcriptional Activators ESX and NF-kB
转录激活剂 ESX 和 NF-kB 的小分子抑制剂
- 批准号:
8444638 - 财政年份:2009
- 资助金额:
$ 4万 - 项目类别:
Small Molecule Inhibitors of the Transcriptional Activators ESX and NF-kB
转录激活剂 ESX 和 NF-kB 的小分子抑制剂
- 批准号:
7699051 - 财政年份:2009
- 资助金额:
$ 4万 - 项目类别:
Small Molecule Inhibitors of the Transcriptional Activators ESX and NF-kB
转录激活剂 ESX 和 NF-kB 的小分子抑制剂
- 批准号:
8032426 - 财政年份:2009
- 资助金额:
$ 4万 - 项目类别:
Chemical Tools for Regulating Eukaryotic Transcription
调节真核转录的化学工具
- 批准号:
6723782 - 财政年份:2002
- 资助金额:
$ 4万 - 项目类别:
Probing Transcriptional Activation at the Molecular Level
在分子水平上探测转录激活
- 批准号:
8065354 - 财政年份:2002
- 资助金额:
$ 4万 - 项目类别:
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