MODULATION OF NEOCORTICAL INTERNEURONAL FUNCTION

描述：（改编自研究者摘要）：升序 脑干和基底前脑的神经递质系统调节 通过直接作用于GABA能抑制剂 中间神经元。有数据表明，乙酰胆碱（ACh）等神经递质 和5-羟色胺（5-HT）可以选择性地激活 抑制性中间神经元通过不同种类的突触后受体和 从而改变皮层回路中的信息流。这些实验 将检查ACh和5-HT对低阈值锋电位（LTS）和快 大鼠视皮层V层GABA能中间神经元数量增多。关于 将使用膜片钳技术获得全细胞记录，以评价 胆碱能和5-羟色胺能受体在不同亚型上的亚型 和突触后反应的性质。的配对的 记录时，单一抑制性突触后电流将在 锥体神经元与胆碱能和5-羟色胺能神经元的突触前效应 将评估激动剂。细胞内标记生物素结合 将使用钙结合蛋白和神经肽的免疫细胞化学 沿着电生理学行为来对中间神经元进行分类以进一步 检验中间神经元亚群被选择性调节的假设 ACh和5-HT。考虑到GABA能抑制网络在神经系统中的重要性， 信息处理和病理生理学过程， 以及这些上行神经递质系统可能 皮质损伤后发生解剖学改变，关于作用的信息 对我们理解正常人的神经系统是至关重要的。 和皮质功能紊乱。

DESCRIPTION: (Adapted from the Investigator's Abstract): Ascending neurotransmitter systems from the brainstem and basal forebrain regulate activities in cortical networks through direct actions on GABAergic inhibitory interneurons. Data suggest that neurotransmitters such as acetylcholine (ACh) and serotonin (5-HT) can selectively activate particular subgroups of inhibitory interneurons through different classes of postsynaptic receptors and thus modify the flow of information in cortical circuits. These experiments will examine effects of ACh and 5-HT on low threshold spike (LTS) and fast spiking aboutS) GABAergic interneurons in layer V of rat visual cortex. about Patch-clamp techniques will be used to obtain whole cell recordings to evaluate the subtypes of cholinergic and serotonergic receptors on different subclasses of interneurons and the nature of the postsynaptic responses. In paired recordings, unitary inhibitory postsynaptic currents will be evoked on pyramidal neurons and presynaptic effects of cholinergic and serotonergic agonists will be assessed. Intracellular labeling with biocytin combined with immunocytochemistry for calcium binding proteins and neuropeptides will be used along with electrophysiological behavior to classify interneurons to further test the hypothesis that subgroups of interneurons are selectively modulated by ACh and 5-HT. Given the importance of the GABAergic inhibitory network in information processing and pathophysiologic processes such as epileptogenesis, and the observation that these ascending neurotransmitter systems may be altered anatomically following cortical lesions, information regarding action of modulators like ACh and serotonin is critical to our understanding of normal and disordered cortical function.