Cystein, Intestinal Thiols and Goblet Cell Development

半胱氨酸、肠硫醇和杯状细胞发育

基本信息

  • 批准号:
    6678652
  • 负责人:
  • 金额:
    $ 29.4万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2003
  • 资助国家:
    美国
  • 起止时间:
    2003-07-01 至 2008-06-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): The proposal originates from our observations of a three to four fold increase in the number of acidomucin-positive goblet cells accompanied by glutathione (GSH) depletion in the small intestinal mucosa of neonatal pigs being nourished parenterally. We hypothesize that the lack of luminal cysteine is the primary insult perceived by the parenterally nourished epithelium, and that the functional endpoint of this nutritional deficiency is a more oxidized intracellular redox potential The identification of this amino acid as a key nutritional factor is based on (a) the high rate of first-pass consumption of dietary cysteine and the low rate of arterial cysteine utilization by the intestine; (b) evidence that cellular redox status is a key determinant of cellular differentiation, and (c) the fact that cysteine is structurally and functionally involved in both intracellular redox status and mucosal defense via its inherent contribution to the thiol buffers GSH and thioredoxin (Trx) on one hand, and the goblet cell-specific secretory mucins and intestinal trefoil factor (ITF) on the other. We hypothesize that parenteral nutrition results in a preferential activation of the mucous cell lineage as well as selective sparing of goblet cells leading to the repopulation (at a reduced rate) of the villus with goblet cells relative to absorptive enterocytes. The alteration in goblet cell development is initiated by hyperoxia that stems from compromised cysteine status. In other words, we hypothesize that goblet cells are the guardians of mucosal redox homeostasis. Our hypothesis will be tested in three specific aims: 1) Determine if a causal relationship exists between epithelial redox status and goblet cell expansion, independent of TPN-associated inflammation. 2) Quantify, in a goblet cell culture model system, the hierarchy of cysteine usage for the synthesis of GSH and Trx versus ITF and secretory mucins, as well as the role of the transsulfuration pathway in the formation of cysteine and sulfate from methionine, as affected by apical versus basal provision of cysteine, and in response to graded levels of hyperoxia. Simultaneously, determine if the source (inorganic vs. cysteine-derived) and flux of sulfate used for sulfomucin biosynthesis is affected by cysteine availability and hyperoxic stress. 3) Test the hypothesis that Cysl31 and/or Cys165, found within the activation domain of Cdx2, are redox sensitive and upon post-translational modification alter the specificity of Cdx2 transcriptional regulation.
描述(由申请方提供):该提议源于我们观察到肠外营养新生猪小肠粘膜中酸性粘蛋白阳性杯状细胞数量增加3 - 4倍,伴有谷胱甘肽(GSH)耗竭。我们假设缺乏鲁米那半胱氨酸是肠外营养上皮细胞感受到的主要损伤,并且这种营养缺乏的功能终点是更氧化的细胞内氧化还原电位。将这种氨基酸鉴定为关键营养因子是基于(a)膳食半胱氨酸的高首过消耗率和肠动脉半胱氨酸利用率低;(B)细胞氧化还原状态是细胞分化的关键决定因素的证据,和(c)半胱氨酸在结构和功能上参与细胞内氧化还原状态和粘膜防御的事实,一方面通过其对巯基缓冲剂GSH和硫氧还蛋白(Trx)的固有贡献,另一方面通过其对杯状细胞特异性分泌粘蛋白和肠三叶因子(ITF)的固有贡献。我们假设肠外营养会导致粘液细胞谱系的优先激活以及杯状细胞的选择性保留,从而导致相对于吸收性肠细胞,绒毛中杯状细胞的重新繁殖(速度降低)。杯状细胞发育的改变是由半胱氨酸状态受损引起的高氧引起的。换句话说,我们假设杯状细胞是粘膜氧化还原稳态的监护人。我们的假设将在三个特定的目标进行测试:1)确定上皮氧化还原状态和杯状细胞扩张之间是否存在因果关系,独立于TPN相关炎症。 2)在杯状细胞培养模型系统中,量化半胱氨酸用于合成GSH和Trx与ITF和分泌粘蛋白的层次结构,以及转硫途径在从甲硫氨酸形成半胱氨酸和硫酸盐中的作用,受顶部与底部的影响。半胱氨酸的供应,以及对高氧分级水平的响应。同时,确定用于磺粘蛋白生物合成的硫酸盐的来源(无机与半胱氨酸衍生)和通量是否受半胱氨酸可用性和高氧应激的影响。 3)测试Cdx 2激活结构域中发现的Cysl 31和/或Cysl 65是氧化还原敏感的并且在翻译后修饰时改变Cdx 2转录调控的特异性的假设。

项目成果

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Rex Gaskins其他文献

Rex Gaskins的其他文献

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{{ truncateString('Rex Gaskins', 18)}}的其他基金

Diet modulation of bacterial sulfur & bile acid metabolism and colon cancer risk
细菌硫的饮食调节
  • 批准号:
    9751249
  • 财政年份:
    2016
  • 资助金额:
    $ 29.4万
  • 项目类别:
Diet modulation of bacterial sulfur & bile acid metabolism and colon cancer risk
细菌硫的饮食调节
  • 批准号:
    9094223
  • 财政年份:
    2016
  • 资助金额:
    $ 29.4万
  • 项目类别:
FRET-based Biosensors to Monitor Redox in Cell Cycle Regulation
基于 FRET 的生物传感器可监测细胞周期调节中的氧化还原
  • 批准号:
    8305728
  • 财政年份:
    2010
  • 资助金额:
    $ 29.4万
  • 项目类别:
FRET-based Biosensors to Monitor Redox in Cell Cycle Regulation
基于 FRET 的生物传感器可监测细胞周期调节中的氧化还原
  • 批准号:
    7946135
  • 财政年份:
    2010
  • 资助金额:
    $ 29.4万
  • 项目类别:
FRET-based Biosensors to Monitor Redox in Cell Cycle Regulation
基于 FRET 的生物传感器可监测细胞周期调节中的氧化还原
  • 批准号:
    8129427
  • 财政年份:
    2010
  • 资助金额:
    $ 29.4万
  • 项目类别:
Cystein, Intestinal Thiols and Goblet Cell Development
半胱氨酸、肠硫醇和杯状细胞发育
  • 批准号:
    6911639
  • 财政年份:
    2003
  • 资助金额:
    $ 29.4万
  • 项目类别:
Cystein, Intestinal Thiols and Goblet Cell Development
半胱氨酸、肠硫醇和杯状细胞发育
  • 批准号:
    7087054
  • 财政年份:
    2003
  • 资助金额:
    $ 29.4万
  • 项目类别:
Cystein, Intestinal Thiols and Goblet Cell Development
半胱氨酸、肠硫醇和杯状细胞发育
  • 批准号:
    7261250
  • 财政年份:
    2003
  • 资助金额:
    $ 29.4万
  • 项目类别:
Cystein, Intestinal Thiols and Goblet Cell Development
半胱氨酸、肠硫醇和杯状细胞发育
  • 批准号:
    6762359
  • 财政年份:
    2003
  • 资助金额:
    $ 29.4万
  • 项目类别:
ENVIRONMENTAL MODULATION OF INTESTINAL SULFIDOGENS AND I
肠道硫化物和 I 的环境调节
  • 批准号:
    6178806
  • 财政年份:
    1999
  • 资助金额:
    $ 29.4万
  • 项目类别:

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基于cysteine代谢在内皮损伤中的作用探讨其在SARSCoV-2感染的致病机理及可能的治疗机制
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The Bioorganic Chemistry of Electrophilic Sulfur in Cysteine Post-Translational Modifications
半胱氨酸翻译后修饰中亲电硫的生物有机化学
  • 批准号:
    2313438
  • 财政年份:
    2023
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    $ 29.4万
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    Standard Grant
Targeting SHP-1 through a newfound metabolite-regulated cysteine activation site
通过新发现的代谢物调节的半胱氨酸激活位点靶向 SHP-1
  • 批准号:
    10802649
  • 财政年份:
    2023
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  • 项目类别:
The role of NAGK cysteine deprotonation in nutrient stress and cancer progression
NAGK 半胱氨酸去质子化在营养应激和癌症进展中的作用
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    10652823
  • 财政年份:
    2023
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Regulation of hepatic lysine N-acetylation by cysteine proximity due to alcohol toxicity
酒精毒性导致的半胱氨酸接近对肝脏赖氨酸 N-乙酰化的调节
  • 批准号:
    10752320
  • 财政年份:
    2023
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    $ 29.4万
  • 项目类别:
Mechanochemical peptide synthesis between chiral α-amido nitrile and cysteine
手性α-酰胺腈和半胱氨酸的机械化学肽合成
  • 批准号:
    23K19234
  • 财政年份:
    2023
  • 资助金额:
    $ 29.4万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
O-glycosylation of cysteine-rich modules
富含半胱氨酸的模块的 O-糖基化
  • 批准号:
    10559833
  • 财政年份:
    2023
  • 资助金额:
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  • 项目类别:
Investigating Autophagy-related Cysteine Protease Atg4a in Drosophila melanogaster Models of Cancer
研究果蝇癌症模型中与自噬相关的半胱氨酸蛋白酶 Atg4a
  • 批准号:
    495180
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    2023
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    $ 29.4万
  • 项目类别:
Studies on the impact of acetyl-cysteine on metabolism
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    10574934
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Targeting cysteine susceptibility in glioblastoma
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    10507157
  • 财政年份:
    2022
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Enabling first-in-class site-selective protein modification strategies beyond cysteine
实现半胱氨酸以外的一流位点选择性蛋白质修饰策略
  • 批准号:
    2726181
  • 财政年份:
    2022
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  • 项目类别:
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