Role of intestinal SIRT1 during liver regeneration

肝脏是一个自我更新的器官，通过一个迷人而高度复杂的过程，即肝脏再生，在体内平衡中维持其生理活动。这一过程还可以使肝脏在受到（药物/毒素）攻击或手术切除后，在啮齿动物中10天内完全恢复其质量和功能，在人类中6-8周。肝脏是人体的主要代谢器官，在这里合成必需的代谢物，为全身提供葡萄糖、脂质、蛋白质和胆汁酸。肝脏不能单独完成这项工作，它需要肠道从我们所吃的食物中提取营养，为肝脏提供代谢活动所需的基础材料。不幸的是，我们的身体面临着不断的挑战，其中一些涉及肝脏功能的丧失，需要在不利的条件下再生。为了更好地了解肝脏如何再生，将使我们能够提出保护和改善肝脏代谢功能和整体全身健康的策略。该项目的目的是确定肝脏再生的机制，更具体地说，确定特定肠道代谢调节因子在这一过程中的作用。在体内的技术将学习，包括动物处理和复杂的外科手术，以及原代细胞的分离体外研究。将培训细胞生物学方法，包括qPCR核酸分析和western blot蛋白质分析，ELISA和免疫组织化学。如何用FACS表征免疫反应也将被学习。

The liver is a self-renewing organ that maintains its physiological activity during homeostasis via a fascinating and highly complex process named liver regeneration. This process also allows the liver to fully restore its mass and function after being challenged (with drugs/toxins) or surgically resected within 10 days in rodents and 6-8 weeks in humans. The liver is the main metabolic organ of the body where the synthesis of essential metabolites occurs, feeding the whole body with glucose, lipids, proteins and bile acids. The liver cannot do this alone and needs the intestine to extract nutrients from the food we eat, providing the liver with the building blocks needed for its metabolic activity. Unfortunately, our body faces constant challenges, some involving the loss of function of the liver that needs to regenerate in unfavourable conditions. To gain a better understanding of how the liver regenerates will enable us to propose strategies to preserve and improve liver metabolic function and overall whole-body health. The aim of this project is to define the mechanisms underpinning liver regeneration and more specifically, to determine the role of specific intestinal metabolic regulators in this process. In vivo techniques will be learned including animal handling and complex surgical procedures, as well as isolation of primary cells for in vitro studies. There will be training in cellular biology methodologies including, analysis of nucleic acids by qPCR and proteins by western blot, ELISA and immunohistochemistry. How to characterise the immune response by FACS will also be learned.