Role of intestinal SIRT1 during liver regeneration

肠道SIRT1在肝再生过程中的作用

• 批准号: 2244124
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2244124
• 关键词: Role; intestinal; SIRT1; during; liver

The liver is a self-renewing organ that maintains its physiological activity during homeostasis via a fascinating and highly complex process named liver regeneration. This process also allows the liver to fully restore its mass and function after being challenged (with drugs/toxins) or surgically resected within 10 days in rodents and 6-8 weeks in humans. The liver is the main metabolic organ of the body where the synthesis of essential metabolites occurs, feeding the whole body with glucose, lipids, proteins and bile acids. The liver cannot do this alone and needs the intestine to extract nutrients from the food we eat, providing the liver with the building blocks needed for its metabolic activity. Unfortunately, our body faces constant challenges, some involving the loss of function of the liver that needs to regenerate in unfavourable conditions. To gain a better understanding of how the liver regenerates will enable us to propose strategies to preserve and improve liver metabolic function and overall whole-body health. The aim of this project is to define the mechanisms underpinning liver regeneration and more specifically, to determine the role of specific intestinal metabolic regulators in this process. In vivo techniques will be learned including animal handling and complex surgical procedures, as well as isolation of primary cells for in vitro studies. There will be training in cellular biology methodologies including, analysis of nucleic acids by qPCR and proteins by western blot, ELISA and immunohistochemistry. How to characterise the immune response by FACS will also be learned.

肝脏是一个自我更新的器官，通过一个迷人的和高度复杂的过程，即肝再生，在体内平衡期间维持其生理活性。这一过程还允许肝脏在受到挑战（药物/毒素）或手术切除后在啮齿动物中10天内和人类中6-8周内完全恢复其质量和功能。肝脏是身体的主要代谢器官，在那里发生必需代谢物的合成，为整个身体提供葡萄糖，脂质，蛋白质和胆汁酸。肝脏不能单独做到这一点，需要肠道从我们吃的食物中提取营养物质，为肝脏提供代谢活动所需的构建模块。不幸的是，我们的身体面临着不断的挑战，一些涉及肝脏功能的丧失，需要在不利的条件下再生。为了更好地了解肝脏如何再生，我们将能够提出保护和改善肝脏代谢功能和整体全身健康的策略。该项目的目的是确定支持肝再生的机制，更具体地说，确定特定肠代谢调节剂在这一过程中的作用。将学习体内技术，包括动物处理和复杂的外科手术，以及分离原代细胞进行体外研究。将有细胞生物学方法的培训，包括通过qPCR和蛋白质印迹，ELISA和免疫组织化学分析核酸。还将学习如何通过FACS检测免疫反应。