课题基金 / 基金详情

MESSENGER RNA DECAY OF IMMEDIATE EARLY GENES

MESSENGER RNA DECAY OF IMMEDIATE EARLY GENES
早期基因的信使 RNA 衰变
批准号：
6603884
负责人：
Ann-Bin Shyu
金额：
$27.49万
依托单位：
UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
项目状态：
已结题
起止时间：
1991-07-01 至 2004-06-30
来源：
https://reporter.nih.gov/project-details/6603884
关键词：
RNA binding protein RNase protection assay antisense nucleic acid cell cycle chemical stability gene expression genetic translation heterogeneous nuclear ribonucleoprotein messenger RNA molecular cloning mutant northern blottings nucleic acid metabolism nucleic acid probes nucleic acid reconstitution nucleic acid sequence nucleic acid structure posttranscriptional RNA processing protein purification protein sequence protooncogene regulatory gene reporter genes site directed mutagenesis

项目摘要

项目成果

Ann-Bin Shyu的其他基金

相似基金

相关文献

中文摘要
翻译
点击翻译按钮获取中文摘要
英文摘要
This proposal by Dr. Shyu is a continuation of his work from the previous funding period. He has made significant progress and contributions to the understanding of ARE-mediated mRNA turnover and appears to have all the groundwork to continue to do so. The proposal investigates the role of hnRNP D (also known as AUF1) in the degradation of ARE-containing mRNAs and how post-translational modifications and associated proteins influence this activity. The specific aims below are designed to obtain answers to the following questions: 1. What are the roles played by stress-activated signaling pathways and the ubiquitin-proteasome pathway in ARE-directed mRNA turnover? 2. What is the ARE/protein decay complex necessary for ARE-dependent mRNA turnover? 3. What structural features of hnRNP D are necessary for its role as a destabilizing protein in ARE-mediated mRNA decay in vivo? 4. How does the ARE exert its destabilizing function? The investigator proposes to address this question by employing in vitro RNA decay systems to dissect and characterize the ARE-targeted mRNA decay.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Regulation of Messenger RNA Turnover in Mammalian Cells
Regulation of Messenger RNA Turnover in Mammalian Cells
Translational Regulation in Bronchial Epithelial Cells
Translational Regulation in Bronchial Epithelial Cells
海外基金