Core--molecular biomarkers facility

描述(由申请人提供) 分子生物标记物核心的主要目标是识别 形成显著关联的基因表达变化模式 自闭症在人类群体中存在，或者与特定的 动物模型中的环境因素。核心将利用这两个 支持人类和动物的广谱和聚焦微阵列 研究项目I-III中描述的研究。为了满足这些需求，Core II将提供统一的处理、提取和分离来自 由中心调查人员生成的组织样本。此外，核心 将使用人类和小鼠基因组文库来创建有针对性的 微阵列。S核心工作的一个重要组成部分将是 进一步开发微阵列数据的分析方法，以努力 两者都可以更好地定义和减少与 微阵列信息的解释。

DESCRIPTION (provided by applicant) The principal goal of the Molecular Biomarkers Core is to identify patterns of altered gene expression that form a significant association with autism in human populations, or which are coupled to specific environmental factors in animal models. The core will utilize both broad-spectrum and focused microarrays to support the human and animal studies described in Research Projects I-III. To meet these needs, Core II will provide uniform handling, extraction, and isolation of mRNA from tissue samples generated by Center investigators. In addition, the core will use both human and mouse genomic libraries to create targeted microarrays. An essential component of the Core s efforts will be to further develop methods of analysis for microarray data in an effort to both better define, and reduce the errors associated with the interpretation of microarray information.