PURCHASE OF A VARIAN INOVA 600 MHz NMR SPECTROMETER

购买 VARIAN INOVA 600 MHz 核磁共振波谱仪

• 批准号: 6440324
• 负责人: ERIC T. KOOL
• 金额: $ 50万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-15 至 2003-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440324
• 关键词: biomedical equipment purchase; enzyme structure; nuclear magnetic resonance spectroscopy; protein protein interaction; protein structure function; structural biology

We propose to purchase a 600 MHz Varian Inova Nuclear Magnetic Resonance spectrometer for the Chemistry Department at Stanford University. This instrument will enable researchers to determine high precision structures of macromolecules including proteins, nucleic acids, and protein-protein, protein-ligand, and protein-nucleic complexes. The instrument will directly support the research groups of the P.I. and co-P.I.s (representing approximately 80 researchers), and it is expected that they will be the major users, but will also be available to the Department as a whole (approximately 350 researchers). The instrument will be placed in existing space in the NMR lab in the Chemistry Department, and will be maintained by 2 full-time Ph.D. NMR staffpersons and by 5 graduate assistants. The research proposed specifically identifies the need for a devoted high field NMR instrument for biomolecular structure analysis. Such research includes structural studies of enzymes that synthesize natural product antibiotics, anticancer drugs and the interaction with their target enzymes, a polyketide and derivatives involved in apoptosis, protein-protein and protein-ligand interactions involved in immune response, DNA helices involved in DNA replication and DNA repair, and RNA relating to Hepatitis C virus. All of the P.I.s' research programs are currently supported by NIH and most by NSF as well. Little of this work can be accomplished on current equipment of the Chemistry Department, and all four P.I.s have been forced recently to go elsewhere to carry out needed structural studies, hampering their research. The specific instrumental package we propose to purchase includes the Oxford 14.1 T magnet, as well as supershims, two Fullband RF channels with three total RF channels, waveform generators for each channel, a variable temperature unit, pulsed field gradients, a Sun workstation, and a cryo probe.

我们建议为斯坦福大学化学系购买一台600 MHz Varian Inova核磁共振光谱仪。 该仪器将使研究人员能够确定高精度的大分子结构，包括蛋白质，核酸，蛋白质-蛋白质，蛋白质-配体和蛋白质-核酸复合物。 该仪器将直接支持P.I.的研究小组。和共同参与者（代表大约80名研究人员），预计他们将是主要用户，但也将提供给整个部门（大约350名研究人员）。 仪器将放置在化学系NMR实验室的现有空间内，并由2名全职博士进行维护。NMR工作人员和5名研究生助理。这项研究提出了专门确定需要一个专门的高场核磁共振仪器的生物分子结构分析。 这些研究包括合成天然产物抗生素的酶的结构研究，抗癌药物及其与靶酶的相互作用，参与细胞凋亡的聚酮化合物和衍生物，参与免疫反应的蛋白质-蛋白质和蛋白质-配体相互作用，参与DNA复制和DNA修复的DNA螺旋，以及与丙型肝炎病毒有关的RNA。 所有的私人研究所的研究项目目前都得到了NIH的支持，大部分也得到了NSF的支持。这些工作在化学系现有的设备上几乎无法完成，所有四名PI最近都被迫去其他地方进行必要的结构研究，阻碍了他们的研究。我们建议购买的具体仪器包包括Oxford 14.1 T磁铁以及超级垫片、两个全频带RF通道（总共三个RF通道）、每个通道的波形发生器、可变温度单元、脉冲场梯度、Sun工作站和低温探头。