APPLIED BISYSTEMS PRISM 3100 GENETIC ANALYZER

APPLIED BISYSTEMS PRISM 3100 遗传分析仪

• 批准号: 6440153
• 负责人: EDWARD K CHAN
• 金额: $ 15.48万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440153
• 关键词: biomedical equipment purchase; nucleic acid quantitation /detection; nucleic acid sequence

DNA sequencing is an integral component of modern molecular biology essential to many biomedical research scientists on a regular basis. This application request for funding of a new ABI DNA analyzer model 3100 to be installed and managed by the established departmental Core Lab in the Department of Molecular and Experimental Medicine (MEM), The Scripps Research Institute (TSRI). MEM is currently one of the larger of the nine departments within TSRI and is composed of over 55 active research faculty members and more than 90 postdoctoral fellows and research associates. The MEM Core Laboratory, established about 12 years ago, has been managed by the P.I. and two technicians. The DNA sequencing service provided by the MEM Core is available primarily for, but not restricted to, the MEM department which is located in a 20-month old new building across the North Torrey Pines Road on the east side of the TSRI campus. Over the past 6 years, the Core's DNA sequencing service has been made available to investigators associated with the Multipurpose Arthritis and Musculoskeletal Disease Center (MAMDC) for San Diego through funding from NIAMS, NIH. As the MAMDC program is terminating at NIAMS, continuing funding for the sequencing service has been submitted under the new NIAMS program Rheumatic Diseases Core Centers (RDCC). The proposed RDCC will provide sequencing service, through the MEM Core to investigators involved in studies of rheumatic diseases in UCSD and other institutions in San Diego. The proposed ABI 3100 DNA analyzer exemplifies second-generation application of capillary electrophoresis for sequence analysis. Recent advances in the DNA analyzer technology include speed, throughput, and labor saving as a result of the enhancement in automation, all of which are highly desirable for the MEM Core sequencing service. The new analyzer will largely replace the current ABI 377 sequencer with the exception for special DNA sequencing needs in a core facility environment. The annual maintenance cost for the proposed ABI 3100 will be supported by departmental funds. This instrument will be an important asset to serve well in furthering the progress of many NIH- funded research in the MEM Department and the proposed RDCC.

DNA测序是现代分子生物学的一个组成部分，对许多生物医学研究科学家来说是必不可少的。本申请要求为新的ABI DNA分析仪型号3100提供资金，该分析仪将由斯克里普斯研究所（TSRI）分子和实验医学系（MEM）的既定部门核心实验室安装和管理。MEM目前是TSRI内九个部门中较大的一个，由超过55名活跃的研究教师和90多名博士后研究员和研究助理组成。MEM核心实验室成立于12年前，由P.I.管理。两个技术员。由MEM核心提供的DNA测序服务主要适用于但不限于MEM部门，该部门位于TSRI校园东侧的North Torrey Pines Road对面的一栋20个月的新大楼内。在过去的6年中，核心的DNA测序服务已提供给与圣地亚哥多功能关节炎和肌肉骨骼疾病中心（MAMDC）相关的研究人员，由NIAMS，NIH资助。随着MAMDC计划在NIAMS终止，测序服务的持续资金已在新的NIAMS计划风湿病核心中心（RDCC）下提交。拟议中的RDCC将通过MEM核心向加州大学圣地亚哥分校和圣地亚哥其他机构参与风湿性疾病研究的研究人员提供测序服务。ABI 3100 DNA分析仪是毛细管电泳在序列分析中的第二代应用。DNA分析仪技术的最新进展包括自动化增强带来的速度、吞吐量和劳动力节省，所有这些都是MEM Core测序服务非常需要的。新的分析仪将在很大程度上取代目前的ABI 377测序仪，但核心设施环境中的特殊DNA测序需求除外。拟议的ABI 3100每年的保养费用，将由部门拨款支付。这一工具将是一个重要的资产，有助于促进MEM部门和拟议的RDCC中许多NIH资助的研究的进展。