Typhoon 8600 Variable Mode Imager Workstation

敏感、定量和高效的数据成像/分析对科学研究的各个方面都至关重要。该提案要求资金购买分子动力台风8600可变模式成像工作站。该成像工作站将允许在一个高通量系统中进行多色荧光、磷光成像和化学发光检测，以满足位于该部门的16个研究小组的需求。微生物学与分子生物学遗传学，其中许多将每天使用该仪器。该仪器支持的研究领域很广，包括复制、转录、mRNA加工、信号转导、细胞信号传导和病原体-宿主相互作用。由于该成像系统的用户将搬到新的场外研究机构，因此在其新建筑（ICPH）中将没有类似的仪器，该建筑位于新泽西州纽瓦克经济落后地区的新研究孵化区（称为科学园）。这一举措使得购买该设备成为这些生产性研究项目成功搬迁的关键组成部分，并将为纽瓦克科学园计划的发展做出重要贡献。该设备将由J. Wilusz博士监督，并由部门工作人员维护，作为新的ICPH大楼新的多用户成像设施的关键组成部分。这些人在维护高端设备服务部门研究人员方面有着良好的记录。影像设施人员的职责包括操作和指导使用仪器、监察仪器的使用情况，以及为其他科学园研究人员制订日后共用仪器的协议。ImageQuant和Excel软件的培训将由在职IT专家提供，以确保所有研究小组都能充分利用该设备。预计台风成像仪不仅将提高确定的用户群体的研究生产力，而且还将允许开发没有仪器就不可能实现的新的实验方法。

Sensitive, quantitative and efficient data imaging/analysis is vital for all facets of scientific research. This proposal requests funds to purchase a Molecular Dynamics Typhoon 8600 Variable Mode Imager Workstation. This imaging workstation will allow multi-color fluorescence, phosphorimaging and chemiluminescence detection in a single high throughput system to serve the needs of 16 research groups located in the Dept. of Microbiology & Mol. Genetics, many of which will use the instrument on a daily basis. The areas of research supported by this instrument are broad, including replication, transcription, mRNA processing, signal transduction, cell signaling and pathogen- host interactions. Since the users of this imaging system will be moving to a new off-site research facility, no comparable instrumentation will be available in their new building (ICPH) that is located in a new research incubator area called Science Park in an economically disadvantaged section of Newark, NJ. This move makes purchase of this device a key component of the successful relocation of these productive research programs, and would represent an important contribution to the development of the Newark Science Park Initiative. The equipment will be supervised by Dr. J. Wilusz and maintained by departmental staff as a key component of a new multi-user imaging facility in the new ICPH building. These individuals have a proven rack-record in maintaining high-end equipment servicing departmental researchers. Duties of Imaging Facility Staff will include operation and instruction on use of the device, monitoring usage and developing future sharing agreements for other Science Park Researchers. Training in ImageQuant and Excel software will be provided by an on-staff IT Specialist to ensure the device will be used to its full potential by all research groups. It is anticipated that the Typhoon Imager will not only increase research productivity of the identified user group, but will also allow the development of novel experimental approaches that would not be possible without the instrument.