Analysis of Biomolecular Associations

生物分子关联分析

• 批准号: 6440234
• 负责人: Michael D. Brenowitz
• 金额: $ 27万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440234
• 关键词: CD1 molecule; DNA directed RNA polymerase; antiviral agents; binding sites; biomedical equipment purchase; gap junctions; glutathione transferase; human immunodeficiency virus; immunology; membrane channels; neurosciences; protein binding; protein protein interaction; proteomics; surface plasmon resonance; transferrin receptor; ultracentrifugation

DESCRIPTION (provided by the applicant): Ligand binding and macromolecular associations are generally grouped under the rubric "Reversible Associations" to denote their dependence upon noncovalent interactions that are in dynamic equilibrium in solution. A surface plasmon resonance instrument (BIAcore) is requested to join our existing analytical ultracentrifuge in order to establish a shared facility for the quantitative analysis of reversible associations. The research interests of the application participants encompass a broad range of important biomedical problems including assembly of gene transcription initiation and regulatory complexes, anti-HIV drug design, ligand binding and allostery, immunology and neuroscience. These instruments will be housed in a location convenient to the users' laboratories and will be accessible to the entire Einstein research community through the Laboratory for Macromolecular Analysis & Proteomics. The PI and other participants in this proposal have a proven track record in the organization and administration of shared resources. The institution provides substantial support to the Laboratory for Macromolecular Analysis & Proteomics including support for the instrument specialist who is overseeing use of the existing analytical ultracentrifuge and who will take charge of the new instrument requested in this grant.

描述(申请人提供)：配体结合和大分子 协会一般归为“可逆协会”类别。 表示它们对处于动态状态的非共价相互作用的依赖性 溶液中的平衡。一种表面等离子体共振仪(Biacore)是 要求加入我们现有的分析超速离心机，以便建立 用于可逆关联的定量分析的共享设施。这个 应用程序参与者的研究兴趣包括广泛的 包括基因转录组装在内的重要生物医学问题 启动和调节复合体，抗HIV药物设计，配体结合和 变构、免疫学和神经科学。这些仪器将被安置在一个 位置方便用户的实验室，并将可进入 整个爱因斯坦研究社区通过大分子实验室 分析与蛋白质组学。PI和本提案的其他参与者有一个 在组织和管理共享资源方面有良好的记录。 该机构为实验室提供了大量的支持 大分子分析和蛋白质组学，包括对仪器的支持 负责监督现有分析超速离心机使用的专家 谁将负责这笔赠款中要求的新文书。