Analysis of Biomolecular Associations

生物分子关联分析

• 批准号: 6440234
• 负责人: Michael D. Brenowitz
• 金额: $ 27万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440234
• 关键词: CD1 molecule; DNA directed RNA polymerase; antiviral agents; binding sites; biomedical equipment purchase; gap junctions; glutathione transferase; human immunodeficiency virus; immunology; membrane channels; neurosciences; protein binding; protein protein interaction; proteomics; surface plasmon resonance; transferrin receptor; ultracentrifugation

DESCRIPTION (provided by the applicant): Ligand binding and macromolecular associations are generally grouped under the rubric "Reversible Associations" to denote their dependence upon noncovalent interactions that are in dynamic equilibrium in solution. A surface plasmon resonance instrument (BIAcore) is requested to join our existing analytical ultracentrifuge in order to establish a shared facility for the quantitative analysis of reversible associations. The research interests of the application participants encompass a broad range of important biomedical problems including assembly of gene transcription initiation and regulatory complexes, anti-HIV drug design, ligand binding and allostery, immunology and neuroscience. These instruments will be housed in a location convenient to the users' laboratories and will be accessible to the entire Einstein research community through the Laboratory for Macromolecular Analysis & Proteomics. The PI and other participants in this proposal have a proven track record in the organization and administration of shared resources. The institution provides substantial support to the Laboratory for Macromolecular Analysis & Proteomics including support for the instrument specialist who is overseeing use of the existing analytical ultracentrifuge and who will take charge of the new instrument requested in this grant.

描述（由申请人提供）：配体结合和大分子 协会通常被归类为“可逆协会” 以表示它们对非共价相互作用的依赖性， 溶液平衡本发明公开了一种表面等离子体共振仪器（BIAcore）， 要求加入我们现有的分析超级计算机，以建立 一个共享的设施的定量分析可逆协会。的 申请者的研究兴趣包括广泛的 重要的生物医学问题，包括基因转录的组装 起始和调节复合物，抗HIV药物设计，配体结合和 变构学、免疫学和神经科学。这些仪器将安装在一个 位置方便用户的实验室，并将访问 整个爱因斯坦研究社区通过实验室的高分子 分析和蛋白质组学。PI和本提案的其他参与者 在组织和管理共享资源方面有良好的业绩记录。 该机构为实验室提供大量支持， 大分子分析和蛋白质组学，包括仪器支持 负责监督现有分析超级计算机使用的专家， 他将负责本授权书中要求的新仪器。