Analysis of Biomolecular Associations

描述(申请人提供)：配体结合和大分子 协会一般归为“可逆协会”类别。 表示它们对处于动态状态的非共价相互作用的依赖性 溶液中的平衡。一种表面等离子体共振仪(Biacore)是 要求加入我们现有的分析超速离心机，以便建立 用于可逆关联的定量分析的共享设施。这个 应用程序参与者的研究兴趣包括广泛的 包括基因转录组装在内的重要生物医学问题 启动和调节复合体，抗HIV药物设计，配体结合和 变构、免疫学和神经科学。这些仪器将被安置在一个 位置方便用户的实验室，并将可进入 整个爱因斯坦研究社区通过大分子实验室 分析与蛋白质组学。PI和本提案的其他参与者有一个 在组织和管理共享资源方面有良好的记录。 该机构为实验室提供了大量的支持 大分子分析和蛋白质组学，包括对仪器的支持 负责监督现有分析超速离心机使用的专家 谁将负责这笔赠款中要求的新文书。

DESCRIPTION (provided by the applicant): Ligand binding and macromolecular associations are generally grouped under the rubric "Reversible Associations" to denote their dependence upon noncovalent interactions that are in dynamic equilibrium in solution. A surface plasmon resonance instrument (BIAcore) is requested to join our existing analytical ultracentrifuge in order to establish a shared facility for the quantitative analysis of reversible associations. The research interests of the application participants encompass a broad range of important biomedical problems including assembly of gene transcription initiation and regulatory complexes, anti-HIV drug design, ligand binding and allostery, immunology and neuroscience. These instruments will be housed in a location convenient to the users' laboratories and will be accessible to the entire Einstein research community through the Laboratory for Macromolecular Analysis & Proteomics. The PI and other participants in this proposal have a proven track record in the organization and administration of shared resources. The institution provides substantial support to the Laboratory for Macromolecular Analysis & Proteomics including support for the instrument specialist who is overseeing use of the existing analytical ultracentrifuge and who will take charge of the new instrument requested in this grant.