Acquisition of a 600 MHz NMR Cryoprobe

生物技术高级研究中心（CARB）是马里兰大学生物技术研究所（UMBI）和国家标准与技术研究所（NIST）的联合研究所，正在申请资金，以协助购买布鲁克MHz核磁共振（NMR） TXI 5毫米样品直径冷冻探针系统或同等设备。提出的600 MHz核磁共振冷冻探针系统将主要由美国国立卫生研究院资助的CARB研究人员使用多维同核和异核核磁共振方法来研究生物大分子的结构和动力学。目前，CARB的五名主要研究人员的研究项目，包括主要的核磁共振用户组，将从拟议的600 MHz核磁共振冷冻探针系统的收购中显著受益，包括：1)膜靶向视觉钙传感器（Ames）；2)枯草杆菌（Subtilisin）蛋白折叠与稳定性；3) RNA茎环亲吻复合物（Marino）的结构基础；4)蛋白质结构和功能的基因组序列：来自核磁共振波谱（Orban）的结构数据；5)视觉视紫红质的折叠和组装（Ridge/Brabazon）。拟议的600 MHz冷冻探针系统将允许以比传统600 MHz核磁共振系统高约4倍的灵敏度获取核磁共振数据。因此，我们预计它将为样品/实验提供获取核磁共振数据的可能性，这些数据目前由于核磁共振相关性的固有弱点、低溶解度和/或样品可用性有限而无法测量。此外，对于传统样品/实验，它将显着减少大约16倍的测量时间。，从而加快了NIH资助的研究项目。我们的建议有重要的考虑因素。首先，目前UMBI系统或NIST都无法使用600 MHz核磁共振冷冻探针系统。因此，拟议的仪器不仅将成为CARB研究人员的宝贵资源，而且也将成为UMBI其他四个研究中心（即人类病毒学研究所（IHV），农业生物技术中心（CAB），海洋生物技术中心（COMB）和医学生物技术中心MDC）的科学家以及可能需要在生物大分子研究中使用其独特能力的NIST的宝贵资源。其次，提议的600 MHz冷冻探针系统将被安置在我们最近建造的共享核磁共振实验室中，并由CARB承诺的基础设施资金维护。第三，还聘请了一名新的设施经理（Sari）来维护600 MHz核磁共振冷冻探针系统，作为CARB核磁共振设施的一部分。

The Center for Advanced Research in Biotechnology (CARB), a joint research Institute of the University of Maryland Biotechnology Institute (UMBI) and the National Institute of Standards and Technology (NIST), is applying for funds to assist in the purchase of a Bruker MHz Nuclear Magnetic Resonance (NMR) TXI 5 mm sample diameter cryoprobe system, or the equivalent. The proposed 600 MHz NMR cryoprobe system will be used primarily by NIH funded researchers at CARB to investigate the structure and dynamics of biological macromolecules using multi-dimensional homo- and heteronuclear NMR methods. Currently, the research programs of five principle investigators at CARB, who comprise the primary NMR user group, would benefit significantly by the acquisition of the proposed 600 MHz NMR cryoprobe system include: 1) Membrane-targeting Calcium Sensors in Vision (Ames); 2) Protein Folding and Stability of Subtilisin (Bryan); 3) Structural Basis of RNA Stem-Loop Kissing Complexes (Marino); 4) Frm Genomic Sequences in Protein Structure and Function: Structural Data from NMR Spectroscopy (Orban); 5) Folding and Assembly in Visual Rhodopsin (Ridge/Brabazon). The proposed 600 MHz cryoprobe system will allow NMR data to be acquired with a sensitivity that is approximately a factor of 4 greater than conventional 600 MHz NMR systems. We anticipate that it will therefore open up the possibility to acquire NMR data for samples/experiments which are currently impractical to measure due to either inherent weakness of the NMR correlation, low solubility and/or limited sample availability. Additionally, it will dramatically reduce measurement time by approximately a factor of 16 for conventional samples/experiments., thereby accelerating the NIH funded research programs. There are important considerations to our proposal. First, access to a 600 MHz NMR cryoprobe system is currently not available either within the UMBI system or NIST. The proposed instrumentation will therefore not only be a valuable resource for CARB researchers, but also for scientists at the four other research centers of UMBI [i.e. The Institute for Human Virology (IHV), Center for Agricultural Biotechnology (CAB), Center for Marine Biotechnology (COMB) and the Medical Biotechnology enter MDC)] and a NIST who may need to use its unique capabilities in the studies of biological macromolecules. Second, the proposed 600 MHz cryoprobe system will be housed in our recently constructed shared NMR laboratory and maintained with committed infrastructure funds from CARB. Third, a new facility manager (Sari) has also been hired to maintain the 600 MHz NMR cryoprobe system as part of the CARB NMR facility.