CORE--TISSUE CULTURE FACILITY

核心——组织培养设施

• 批准号: 6653318
• 负责人: TOR C. SAVIDGE
• 金额: $ 26.39万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2003-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653318
• 关键词: biomedical facility; gastrointestinal transplantation; laboratory mouse; tissue /cell culture; xenotransplantation

Description (adapted form application): The Tissue Culture/Morphology/Xenograph [sic] Transplant Model Core will provide expertise and services in cell culture, intestinal xenografting and various morphologic techniques, including in situ hybridization, immunohistochemistry and microscopy (light, confocal laser scanning and electron microscopy). The service will also serve to consult individual research projects on use of these and related techniques in the planned experiments, especially with regard to the use of human intestinal xenografts as this experimental tool will be utilized in some capacity by all the projects. It will be a major research resource for the projects by providing a centralized cell culture and microscopy facility. As more of the individual projects are using cell biology approaches, the investigators will require an increasing use of cell culture and morphologic techniques. As part of this re-application, we will expand considerably the options in morphology. We will provide expert advice and service in confocal microscopy, using single, double and triple label options, as well as quantitative analysis. We will also provide an expanded fluorescent microscopy capacity including multiple labels, digital photography and quantitative analysis of digital images. The cores also offer training in specific techniques to members of the individual projects and their laboratories and a valuable resource for intellectual and technical interchange regarding scientific and methodological approaches to morphologic probes (quantitative imaging, etc.).

描述（改编形式申请）：组织培养/形态学/异种移植 [原文如此] 移植模型核心将提供细胞方面的专业知识和服务 培养、肠道异种移植和各种形态学技术，包括 原位杂交、免疫组织化学和显微镜（光、共聚焦 激光扫描和电子显微镜）。该服务还将用于咨询 关于使用这些和相关技术的个人研究项目 计划中的实验，特别是关于使用人体肠道 异种移植作为这种实验工具将被所有人以某种方式使用 项目。它将成为该项目的主要研究资源 提供集中的细胞培养和显微镜设施。随着更多的 个别项目正在使用细胞生物学方法，研究人员将 需要更多地使用细胞培养和形态学技术。作为一部分 在这次重新应用中，我们将大大扩展形态学方面的选项。 我们将使用单一、 双标签和三标签选项以及定量分析。我们还将 提供扩展的荧光显微镜能力，包括多个标记， 数字摄影和数字图像的定量分析。核心还 为各个项目的成员提供特定技术的培训 他们的实验室和宝贵的知识和技术资源 关于形态学的科学和方法论方法的交流 探针（定量成像等）。