CORE--CELL CULTURE AND DNA

核心——细胞培养和DNA

• 批准号: 6660512
• 负责人: Deborah A Driscoll
• 金额: $ 21.13万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6660512
• 关键词: DNA; biomedical facility; cell line; chromosomes; congenital oral /facial /cranial defect; fibroblasts; fluorescent in situ hybridization; hybrid cells; in situ hybridization; lymphocyte; syndrome; tissue /cell culture

Core B will provide tissue culture services including the establishment of cell cultures of EBV-transformed lymphocytes and skin fibroblasts. Slides of metaphase chromosomes will be prepared from harvests of cultured cells for FISH experiments. Genomic DNA will be extracted from various sources and also prepared for PFGE. Somatic cell hybrids will be generated as needed.

核心B将提供组织培养服务，包括 EB病毒转化淋巴细胞和皮肤细胞培养的建立 成纤维细胞中期染色体的载玻片将从以下收获物制备： 用于FISH实验的培养细胞。基因组DNA将从各种 来源，并为PFGE准备。体细胞杂种将产生为 needed.