CORE--MOUSE FACILITY

鼠标核心将支持程序的各个组成部分分为两个 主要方法：a）制备转基因和基因敲除小鼠; 和，B）维持各种突变小鼠品系，用于 建议的研究。 核心的一个主要的初步重点将涉及 建立蛋白4.1敲除小鼠和表达各种 蛋白4.1转基因。 拟议的研究是在体内解剖 蛋白质4.1的生物学功能在项目1，2，3， 和6. 核心将在协助参与者方面发挥核心作用。 在设计的各个方面涉及的小鼠的程序 问题研究 所有的技术步骤，在创造转基因和基因 敲除小鼠（即创建各种DNA构建体并进行 胚胎操作）将作为该核心的一部分进行。 的 核心还将维持和提供地中海贫血小鼠。

The mouse core will support the various components of the program in two major ways: a) the preparation of transgenic and gene knockout mice; and, b) the maintenance of various mutant mouse lines to be used in the proposed studies. A major initial focus of the core will relate to the creation of both protein 4.1 knockout mice and mice expressing various protein 4.1 transgenes. The proposed studies to dissect in vivo the biological functions of protein 4.1 are described in projects 1, 2, 3, and 6. The core will play a central role in assisting the participating programs in designing the various aspects involved in of the murine studies. All the technical steps in creating the transgenic and gene knock- out mice (i.e. creating the various DNA constructs and performing embryo manipulations) will be carried out as part of this core. The core will also maintain and provide thalassemic mice.