Engineering recombinant bacteriophages as multi - tools against intracellular bacterial infections

改造重组噬菌体作为对抗细胞内细菌感染的多功能工具

• 批准号: 2266975
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2266975
• 关键词: Engineering; recombinant; bacteriophages; multi; tools

Bacteriophages are viruses with great specificity for infecting their bacterial hosts, allowing their use as targeted antibacterial agents. Current advances in synthetic biology have enabled the genetic engineering of bacteriophages for a broad range of applications. In this project, we propose to genetically engineer bacteriophage to efficiently enter into human cells to allow for phage-mediated killing of difficult to treat intracellular Escherichia coli K1 infection. A combination of genetics, microbiology, phage biology, synthetic biology, human cell biology and microscopy methods, will enable us to reach the objectives of the project.

噬菌体是具有感染其细菌宿主的高度特异性的病毒，允许其用作靶向抗菌剂。目前合成生物学的进展已经使噬菌体的基因工程能够用于广泛的应用。在这个项目中，我们建议基因工程噬菌体有效地进入人体细胞，使噬菌体介导的杀伤难以治疗的细胞内大肠杆菌K1感染。遗传学、微生物学、噬菌体生物学、合成生物学、人类细胞生物学和显微镜方法的结合将使我们能够实现该项目的目标。