CORE--CDNA ARRAYS

核心——CDNA阵列

• 批准号: 6563946
• 负责人: EDWARD E HARLOW
• 金额: $ 29.18万
• 依托单位: WHITEHEAD INSTITUTE FOR BIOMEDICAL RES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563946
• 关键词: animal genetic material tag; biomedical facility; breast neoplasms; complementary DNA; genetic library; molecular cloning; oncogenes

DESCRIPTION: (Applicant's Description) The overarching goal of this Program Project Grant is to elucidate key aspects of breast development and breast carcinogenesis. The principal research emphasis of the component research programs is a study of the biological function of proteins that play important roles in controlling these processes. The goal of this core is to develop and to make available useful protein expression methodologies that will promote these studies. The work in this core will be divided into two general components. One portion of the work will provide reagents to other investigators in this Program. These services will include the establishment of a bank of approximately 100 key breast genes cloned as full length coding regions and available in all commonly used protein expression vectors (Specific Aim 1). This bank will include genes that are currently known to play a role in yeast development or in the development of breast tumors. In addition, core services will provide normalized cDNA libraries for use in molecular biology, functional, or genetic screens (Specific Aim 2). We also will begin the technology development needed to establish an array of cDNAs in vectors that allow functional testing. The ultimate goal of this work will be to capture all genes expressed in the breast. For the five year period of this grant, our goal will be to isolate 10,000 full length sequences and have them available in vectors that promote functional screens (Specific Aim 3).

描述：（申请人的描述）本计划的总体目标 项目资助是阐明乳房发育和乳房的关键方面 致癌作用构件研究的主要研究重点 该项目是一项研究蛋白质的生物学功能， 在控制这些过程中的作用。这个核心的目标是发展和 提供有用的蛋白质表达方法，以促进 这些研究。 这一核心的工作将分为两大部分。一 这项工作的一部分将为其他研究人员提供试剂， 程序.这些服务将包括建立一个银行， 克隆了大约100个关键乳腺基因作为全长编码区， 所有常用的蛋白质表达载体（特定目标1）。 这个基因库将包括目前已知在酵母中发挥作用的基因 发展或乳腺肿瘤的发展。此外，核心 服务将提供用于分子生物学的标准化cDNA文库， 功能或遗传筛选（特定目标2）。我们也将开始 技术开发需要在载体中建立cDNA阵列， 允许功能测试。这项工作的最终目标将是捕捉 所有基因都在乳房中表达。在这项赠款的五年期间， 我们的目标是分离出一万个全长序列 可用于促进功能筛选的载体（具体目标3）。