CORE--CDNA ARRAYS

描述：(申请者描述)本项目的首要目标 格兰特项目是为了阐明乳房发育和乳房的关键方面 致癌。组件研究的主要研究重点 程序是对发挥重要作用的蛋白质的生物功能的研究 在控制这些过程中扮演的角色。这个核心的目标是开发和 提供有用的蛋白质表达方法，以促进 这些研究。 这一核心的工作将分为两个一般组成部分。一 部分工作将为这一领域的其他调查人员提供试剂 程序。这些服务将包括建立一个 大约100个关键的乳房基因被克隆为全长编码区和 可用于所有常用的蛋白质表达载体(特定目标1)。 这个库将包括目前已知在酵母中发挥作用的基因。 在乳房肿瘤的发生或发展中。此外，核心 服务部门将提供标准化的cDNA文库，用于分子生物学， 功能性筛选或基因筛选(特定目标2)。我们还将开始 技术开发需要在载体中建立一组cDNA， 允许进行功能测试。这项工作的最终目标将是捕获 所有的基因都在乳房中表达。在这笔赠款的五年期间， 我们的目标是分离出10,000个全长序列 可用于推广功能屏幕的载体(特定目标3)。

DESCRIPTION: (Applicant's Description) The overarching goal of this Program Project Grant is to elucidate key aspects of breast development and breast carcinogenesis. The principal research emphasis of the component research programs is a study of the biological function of proteins that play important roles in controlling these processes. The goal of this core is to develop and to make available useful protein expression methodologies that will promote these studies. The work in this core will be divided into two general components. One portion of the work will provide reagents to other investigators in this Program. These services will include the establishment of a bank of approximately 100 key breast genes cloned as full length coding regions and available in all commonly used protein expression vectors (Specific Aim 1). This bank will include genes that are currently known to play a role in yeast development or in the development of breast tumors. In addition, core services will provide normalized cDNA libraries for use in molecular biology, functional, or genetic screens (Specific Aim 2). We also will begin the technology development needed to establish an array of cDNAs in vectors that allow functional testing. The ultimate goal of this work will be to capture all genes expressed in the breast. For the five year period of this grant, our goal will be to isolate 10,000 full length sequences and have them available in vectors that promote functional screens (Specific Aim 3).