CORE--CDNA ARRAYS

核心——CDNA阵列

• 批准号: 6563946
• 负责人: EDWARD E HARLOW
• 金额: $ 29.18万
• 依托单位: WHITEHEAD INSTITUTE FOR BIOMEDICAL RES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563946
• 关键词: animal genetic material tag; biomedical facility; breast neoplasms; complementary DNA; genetic library; molecular cloning; oncogenes

DESCRIPTION: (Applicant's Description) The overarching goal of this Program Project Grant is to elucidate key aspects of breast development and breast carcinogenesis. The principal research emphasis of the component research programs is a study of the biological function of proteins that play important roles in controlling these processes. The goal of this core is to develop and to make available useful protein expression methodologies that will promote these studies. The work in this core will be divided into two general components. One portion of the work will provide reagents to other investigators in this Program. These services will include the establishment of a bank of approximately 100 key breast genes cloned as full length coding regions and available in all commonly used protein expression vectors (Specific Aim 1). This bank will include genes that are currently known to play a role in yeast development or in the development of breast tumors. In addition, core services will provide normalized cDNA libraries for use in molecular biology, functional, or genetic screens (Specific Aim 2). We also will begin the technology development needed to establish an array of cDNAs in vectors that allow functional testing. The ultimate goal of this work will be to capture all genes expressed in the breast. For the five year period of this grant, our goal will be to isolate 10,000 full length sequences and have them available in vectors that promote functional screens (Specific Aim 3).

描述：（申请人的描述）该程序的总体目标 赠款项目是为了阐明乳房发育和乳房的关键方面 致癌作用。组成研究的主要研究重点 程序是对蛋白质的生物学功能的研究 在控制这些过程中的角色。该核心的目的是发展和 为了提供有用的蛋白质表达方法，将促进 这些研究。 该核心中的工作将分为两个一般组成部分。一 工作的一部分将为其他调查人员提供试剂 程序。这些服务将包括建立 大约100个关键乳腺基因克隆为全长编码区域，并且 在所有常用的蛋白质表达载体中可用（特定目标1）。 该银行将包括目前已知在酵母中发挥作用的基因 发育或乳腺肿瘤的发育。另外，核心 服务将提供标准化的cDNA库以用于分子生物学， 功能或遗传筛选（特定目标2）。我们也将开始 技术开发需要在向量中建立各种cDNA 允许功能测试。这项工作的最终目标是捕获 所有在乳房中表达的基因。在这笔赠款的五年期间， 我们的目标是隔离10,000个全长序列并拥有它们 在促进功能屏幕的向量中可用（特定目标3）。