CANCER GENOTYPING WITH CAPILLARY ARRAY ELECTROPHORESIS

这部分计划项目赠款的长期目标是 约翰霍普金斯大学将开发高速、大规模并行的毛细管阵列 可用于高效的电泳设备和方法 癌症基因分型的多重分析。这些研究将提供 可用于癌症诊断应用并将 导致识别与以下相关的特定基因改变 癌症的发展。最初，毛细管阵列扫描仪能够 在不到1%的时间内进行多达1024个DNA扩增产物的电泳分离 一小时将在伯克利建造、评估和优化。一位科学家 来自约翰·霍普金斯的将在伯克利工作，帮助优化这个 并学习如何运行该设备并分析这些数据。 然后这个仪器将被转移到约翰·霍普金斯大学，在那里它将用于 癌症基因分型研究。初始系统将以1/4的速度运行 承载256个样本的能力。它将升级为领先，并运行到 随着更多的样本和ET引物的出现，1024条毛细血管。自定义 ET引物将由一名技术人员在整个工作过程中合成 伯克利。同时，一名研究生将在伯克利大学工作。 4“毛细管阵列电泳仪的微细加工 玻璃晶圆和芯片扫描仪的发展。这些毛细管 阵列电泳板将设计用于运行96个样品和 要执行比传统分离快10倍的分离 上述毛细管阵列装置。样本将被加载到 带有多头移液器和探测器的芯片将随客户提供 芯片扫描仪。如果这些芯片成功，一个芯片扫描仪和芯片 将提供给约翰霍普金斯大学进行癌症基因分型评估 在这笔助学金的第三年学习。CAE微型板应该是 对于实时分析在此期间采集的样本尤其有用 评估肿瘤进展并为下一代提供 在廉价、快速和高通量的癌症基因分型和诊断中 设备。

The long-term objective of this portion of the Program Project Grant with Johns Hopkins is to develop high-speed, massively parallel capillary array electrophoresis apparatus and methods that can be used for efficient multiplex analysis of cancer genotypes. These studies will provide technologies that can be used for cancer diagnostic applications and will lead to the identification of specific gene alterations associated with cancer development. Initially, a capillary array scanner capable of performing up to 1024 DNA amplicon electrophoretic separations in under 1 hour will be constructed, evaluated and optimized at Berkeley. A scientist from John Hopkins will work at Berkeley to aid in the optimization of this apparatus and to learn how to run the apparatus and analyze these data. Then this apparatus will be moved to John Hopkins where it will be used in cancer genotyping studies. The initial system will be operated at 1/4 capacity loading 256 samples. It will be upgraded to lead and run up to 1024 capillaries as more samples and ET primers become available. Custom ET primers will be synthesized throughout this work by a technician at Berkeley. In a parallel effort, a graduate student will work at Berkeley on the microfabrication of capillary array electrophoresis systems on 4" glass wafers and on the development of chip scanners. These capillary array electrophoresis microplates will be designed to run 96 samples and to perform separation that are 10-times faster than the conventional capillary array apparatus mentioned above. Samples will be loaded on the chips with multihead pipetters and detection will be provided with custom chip scanners. If these chips are successful, a chip scanner and chips will be provided to Johns Hopkins for evaluation in cancer genotyping studies in the third year of this grant. The CAE microplates should be particularly valuable for real-time analysis of samples taken during surgery to evaluate tumor progression and to provide the next generation in inexpensive fast and high through-put cancer genotyping and diagnostic devices.