CORE--ISLET CORE

描述（直接取自应用程序）：目标和 具体目的是为参与者提供小鼠、大鼠和猪胰岛组织 PPG的体外研究免疫排斥机制和胰岛 功能，用于基因转移实验和移植。在 此外，胰岛细胞移植的技术将被教导， 研究人员及其实验室成员。 背景：本PPG关注胰岛移植， 预期猪胰岛将被移植到人类， 胰岛素依赖型糖尿病。由于人体胰岛组织严重短缺， 可能需要使用其他物种的胰岛。出于各种 原因，包括现成的可用性，猪似乎特别适合 用于异种移植因此，成年猪胰岛和猪 新生儿胰腺细胞簇（NPCC）将提供给 PPG的参与者。NPCC似乎特别有希望，因为它们 哈代，具有令人印象深刻的生长和分化能力， 似乎特别适合基因改造。大鼠和小鼠 我们亦会为这四个项目提供胰岛。虽然目标是 这项工作是将转基因猪胰岛移植到灵长类动物体内， 首先必须对啮齿动物进行大量的背景工作， 已被证明是研究中使用最广泛的模型 胰岛移植免疫学，以及胰岛生理学和细胞 生物学

Description (taken directly from the application): The objectives and Specific Aims are to provide mouse, rat, and porcine islet tissue to the participants of the PPG for their in vitro studies on immune rejection mechanisms and islet function, for gene transfer experiments, and for transplantation. In addition, the techniques of islet cell transplantation will be taught to investigators and their lab members as required. Background: This PPG is focused upon islet transplantation, with the expectation that porcine islets will be transplanted into humans with IDDM. Because of the serious shortage of human islet tissue, it will probably be necessary to use islets from other species. For a variety of reasons, including ready availability, pigs seem particularly well suited for xenografting. Therefore, both adult porcine islets and porcine neonatal pancreatic cell clusters (NPCCs) will be made available to the participants of the PPG. NPCCs seem particularly promising because they are hardy, have impressive capacity for growth and differentiation, and seem particularly well suited for genetic modification. Rat and mouse islets will also be supplied to the four Projects. Although the goal of this work is to transplant genetically modified pig islets into primates, a large amount of background work must first be carried out with rodent islets, which have proved to be the most widely used models for the study of islet transplantation immunology, as well as islet physiology and cell biology.