CORE--AUTOMATED DNA SEQUENCING FACILITY

核心——自动化DNA测序设备

• 批准号: 6590254
• 负责人: PAUL F. LAMBERT
• 金额: $ 18.34万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6590254
• 关键词: biomedical automation; biomedical facility; nucleic acid sequence

The recently established Automated DNA Sequencing Facility in the McArdle Laboratory for Cancer Research provides scientists access to state-of-the- art machines for rapid DNA sequencing. All five projects of this program- project grant currently make heavy use of this common facility. The projected needs for this facility will increase in future years. Uses include sequence verification of recombinant DNAs, screening of candidate pools from large-scale projects such as yeast two-hybrid and SELEX procedures, and sequence analysis of tumor suppressor genes and oncogenes in experimental animal tumors. Funds are requested to support the costs for running this facility proportional to its use by the projects supported by this program-project grant. Only costs incurred for running samples on the common machines and the maintenance of those machines are requested under this core. All other costs for the preparation of sequencing samples are incurred by individual projects.

最近在麦卡德尔建立的自动DNA测序设施 癌症研究实验室为科学家提供了访问国家的- 用于快速DNA测序的艺术机器。本项目的五个项目- 项目赠款目前大量使用这一共同设施。的 预计今后几年对这一设施的需求将增加。使用 包括重组DNA序列验证、候选物的筛选 来自酵母双杂交和SELEX等大型项目的池 肿瘤抑制基因和癌基因的序列分析 在实验动物肿瘤中。请求提供资金，以支持 根据项目的使用情况， 由这个项目资助。只有运行成本 常见机器上的样本和这些机器的维护是 在这个核心下，编制《公约》的所有其他费用 测序样本由各个项目产生。