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CORE--PROTEIN EXPRESSION FACILITIES

CORE--PROTEIN EXPRESSION FACILITIES
核心——蛋白质表达设施
批准号：
6571151
负责人：
CHIH-LUEH Albert WANG
金额：
$27.63万
依托单位：
BOSTON BIOMEDICAL RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
项目状态：
已结题
起止时间：
2001-12-01 至 2002-11-30
来源：
https://reporter.nih.gov/project-details/6571151
关键词：
Baculoviridae biomedical facility biotechnology gene expression protein engineering recombinant proteins tissue /cell culture transfection /expression vector

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中文摘要
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英文摘要
Description: The Baculovirus Expression System will be used for expression of recombinant proteins in eukaryotic cells This eukaryotic expression system encompasses many of the post-translational modification pathways, allowing the production of recombinant protein which is antigenically, immunogenically, and functionally similar to the native protein (e.g. MLCK, MAPK). Also, the majority of this overproduced protein remains soluble in the insect cells and the viral genome is relatively large (130 kbp) and thus can accommodate large segments of foreign DNA ( e.g. myosin heavy chain, CaD, etc.). The dual-promoter co-expression vector (e.g. p2Bac) permits simultaneous expression of two recombinant proteins (e.g., myosin heavy chain and light chains). This core will serve Projects 1, 2-A and 2-C.
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Regulation of Vascular Smooth Muscle Cytoskeleton
Regulation of Vascular Smooth Muscle Cytoskeleton
Regulation of Vascular Smooth Muscle Cytoskeleton
Regulation of Vascular Smooth Muscle Cytoskeleton
  • 批准号：
    8657513
  • 项目类别：
  • 资助金额：
    $2.18万
  • 财政年份：
    2008
  • 负责人：
    CHIH-LUEH Albert WANG
  • 依托单位：
    BOSTON UNIVERSITY MEDICAL CAMPUS
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