CORE--CELL IMMORTALIZATION AND TRANSFECTION FACILITY

热罗细胞永生化和转染设施，或CIT， 将由麦克杜格尔博士作为项目负责人监督服务核心是 旨在为研究核心的4个科学项目服务，每个项目 项目将需要牙齿特异性和稳定转染的细胞系。的 服务核心将通过基因转移到几个细胞系来产生永生化细胞系。 通过使用逆转录病毒癌基因在单层培养中的牙细胞类型 结构。将分离稳定的细胞系，并进行基因表达谱分析， 将分析细胞外基质的性质。这些细胞系将作为 在研究核心项目的体外启动子研究的资源。的 服务核心还将进行稳定的细胞转染与各种 启动子-报告子结构由各个项目提供。

The GERO Cell Immortalization and Transfection Facility, or CIT, will be supervised by Dr. MacDougall as project leader. The Service Core is designed to serve the 4 scientific projects of the Research Core, and each project will require tooth specific and stably transfected cell lines. The Service Core will produce immortalized cell lines by gene transfer to several dental cell types in monolayer culture, through use of retroviral oncogene constructs. Stable cell lines will be isolated and gene expression profile and nature of extracellular matrix will be analyzed. These cell lines will serve as a resource for in vitro promoter studies in the Research Core projects. The Service Core will also perform stable cell transfections with various promoter-reporter constructs provided by the individual projects.