CRYO CRYSTALLOGRAPHY OF PHOTOCYCLE INTERMEDIATE OF PHOTOACTIVE YELLOW PROTEIN

光活性黄蛋白光循环中间体的冷冻晶体学

• 批准号: 6658618
• 负责人: ULRICH K GENICK
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658618
• 关键词: bacteria; bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

Photoactive Yellow Protein (PYP) is a small, cytosolic photoreceptor of the bacterium Ectothiorhodaspira halophila. Its small size and its ability to undergo the photocycle even in a crystal make it ideal to study structural changes in proteins by time-resolved crystallography. The determination of the structure of the early intermediate would complete the structural determination of all known structural intermediates of PYP.

光敏黄蛋白（PYP）是一种小的细胞质蛋白， 细菌Ectothiorhodaspira halophila的光感受器。 其 体积小，即使在晶体中也能经历光循环 使其成为研究蛋白质结构变化的理想方法， 结晶学 早期构造的确定 中间体将完成所有已知的结构测定 PYP的结构中间体。